sp. Govern Nitrous Oxide Emissions in a Tropical Soil Amended With Residues of Bioenergy Crop.

Organic vinasse, a residue produced during bioethanol production, increases nitrous oxide (NO) emissions when applied with inorganic nitrogen (N) fertilizer in soil. The present study investigated the role of the ammonia-oxidizing bacteria (AOB) community on the NO emissions in soils amended with organic vinasse (CV: concentrated and V: non-concentrated) plus inorganic N fertilizer. Soil samples and NO emissions were evaluated at 11, 19, and 45 days after fertilizer application, and the bacterial and archaea gene (A) encoding the ammonia monooxygenase enzyme, bacterial denitrifier (K, S, and Z) genes and total bacteria were quantified by real time PCR. We also employed a deep A amplicon sequencing approach to evaluate the effect of treatment on the community structure and diversity of the soil AOB community. Both vinasse types applied with inorganic N application increased the total NO emissions and the abundance of AOB. sp. was the dominant AOB in the soil and was correlated with NO emissions. However, the diversity and the community structure of AOB did not change with vinasse and inorganic N fertilizer amendment. The results highlight the importance of residues and fertilizer management in sustainable agriculture and can be used as a reference and an input tool to determine good management practices for organic fertilization.