Zahari R, Halimoon N, Ahmad M F, Ling S K
Laboratory of Sustainable Bioresource Management (BIOREM), Institute of Tropical Forestry and Forest Products (INTROP), Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Department of Environmental Science, Faculty of Environmental Studies, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Int J Anal Chem. 2018 Mar 5;2018:8150610. doi: 10.1155/2018/8150610. eCollection 2018.
and are root rot rubber diseases and these fungi should be kept under control with environmentally safe compounds from the plant sources. Thus, an antifungal compound isolated from was screened for its effectiveness in controlling the growth of these fungi. The antifungal compound isolated from extract was determined through thin layer chromatography (TLC) and nuclear magnetic resonance (NMR) analysis. Each of the DCM extracts was marked as CRD1, CRD2, CRD3, CRD4, CRD5, CRD6, and CRD7, respectively. TLC results showed that all of the extracts peaked with red colour at Rf = 0.61 at 366 nm wavelength, except for CRD7. The CRD4 extract was found to be the most effective against and with inhibition zones of 3.5 and 1.9 mm, respectively, compared to that of other extracts. These extracts, however, were not effective against The CRD4 extract contained ursolic acid that was detected by NMR analysis and the compound could be developed as a biocontrol agent for controlling and Moreover, little or no research has been done to study the effectiveness of in controlling these fungi.
[前文提到的两种病害名称]是根腐橡胶病害，这些真菌应该用来自植物源的环境安全化合物加以控制。因此，对从[植物名称]中分离出的一种抗真菌化合物进行了筛选，以评估其对这些真菌生长的控制效果。通过薄层色谱（TLC）和核磁共振（NMR）分析确定了从[植物名称]提取物中分离出的抗真菌化合物。二氯甲烷提取物的每一部分分别标记为CRD1、CRD2、CRD3、CRD4、CRD5、CRD6和CRD7。TLC结果表明，除CRD7外，所有[植物名称]提取物在366nm波长下Rf = 0.61处均呈现红色峰值。与其他提取物相比，发现CRD4提取物对[两种真菌名称]最有效，其抑菌圈分别为3.5和1.9mm。然而，这些提取物对[另一种真菌名称]无效。通过NMR分析检测到CRD4提取物含有熊果酸，该化合物可开发为控制[两种真菌名称]的生物防治剂。此外，关于[植物名称]在控制这些真菌方面的有效性，几乎没有进行研究。
