Wahab Noor Zarina Abd, Azizul Aziah, Ibrahim Nazlina
Department of Biomedicine, Faculty of Health Sciences, Universiti Sultan Zainal Abidin, Terengganu, Malaysia.
Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan, Bangi, Malaysia.
Iran J Microbiol. 2020 Oct;12(5):460-465. doi: 10.18502/ijm.v12i5.4608.
is generally used to treat many diseases in folklore remedies. The present study is aimed at determining phytochemical constituents, cytotoxicity and antiviral activities for crude extract of the plant.
The whole plant of was extracted using methanol extraction method. Phytochemical qualitative screening was carried out for extract according to standard procedures used to test for the presence of alkaloid, saponin, terpenoid and steroid. Cytotoxicity was assessed using 3-(4,5-dimethylthiazol-2,5-diphenyltetrazolium bromide (MTT) assay. Plaque reduction assays were carried out to evaluate the antiviral activity of extract against herpes simplex virus type 1 (HSV-1). These include post-treatment, pre-treatment and virucidal assays.
extract contain secondary metabolites such as alkaloid, saponin and terpenoid but does not contain steroid. Cytotoxicity screening against Vero cells using MTT assay showed that the CC values for crude extract of was 0.5 mg/mL. The extract prepared from possesses phytochemical compound that was non-cytotoxic to the cell with potential antiviral activity. Plaque reduction assays against herpes simplex virus type 1 (HSV-1) showed that the selective indices (SI = CC / EC) of extract in post-treatment, pre-treatment and virucidal assays were 36, 20 and 4.7 respectively. The results revealed that the extract prepared from possesses phytochemical compound that was non-cytotoxic to the cell with potential antiviral activity.
This study showed that extract has promising potential to be explored as anti-HSV-1 agent regardless of the mode of treatment.
在民间疗法中通常用于治疗多种疾病。本研究旨在确定该植物粗提物的植物化学成分、细胞毒性和抗病毒活性。
采用甲醇提取法提取该植物的全株。根据用于检测生物碱、皂苷、萜类和甾体存在的标准程序，对提取物进行植物化学定性筛选。使用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐（MTT）法评估细胞毒性。进行蚀斑减少试验以评估提取物对1型单纯疱疹病毒（HSV-1）的抗病毒活性。这些试验包括治疗后、治疗前和杀病毒试验。
提取物含有生物碱、皂苷和萜类等次生代谢产物，但不含甾体。使用MTT法对Vero细胞进行细胞毒性筛选表明，该植物粗提物的半数细胞毒性浓度（CC）值为0.5 mg/mL。该植物制备的提取物含有对细胞无细胞毒性且具有潜在抗病毒活性的植物化学化合物。针对1型单纯疱疹病毒（HSV-1）的蚀斑减少试验表明，提取物在治疗后、治疗前和杀病毒试验中的选择性指数（SI = CC / EC）分别为36、20和4.7。结果表明，该植物制备的提取物含有对细胞无细胞毒性且具有潜在抗病毒活性的植物化学化合物。
本研究表明，无论治疗方式如何，该植物提取物都有作为抗HSV-1药物进行探索的潜力。
