Reheman Ayinuer, Aisa Haji Akber, Ma Qing Ling, Nijat Dilaram, Abdulla Rahima
University of Chinese Academy of Sciences, Beijing 100039, China.
Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi, Xinjiang 830011, China.
Evid Based Complement Alternat Med. 2018 Mar 6;2018:1035809. doi: 10.1155/2018/1035809. eCollection 2018.
By merging a high-performance liquid chromatography diode array detector (HPLC-DAD) method with high-performance thin-layer chromatography (HPTLC), an assay was developed for chemical fingerprinting and quantitative analysis of traditional medicine Majun Mupakhi ELA (MME), and constituent compounds were identified using HPLC coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS method. In addition, the antioxidant capacity of MME was assessed based on the ability of components to scavenge radicals using in vitro method. Using a HPLC-DAD method with HPTLC easily validated the chemical fingerprinting results and quantified three characteristic components, namely, gallic acid (1), daidzein (2), and icariin (3), in commercial MMEs. The three compounds presented excellent regression values ( = 0.9999) in the ranges of the test and the method recovery was in the range from 100.49% to 100.68%. The fingerprints had 27 common characteristic peaks, of which 13 were verified by rapid UHPLC-DAD-Q-Orbitrap-MS analysis. In vitro antioxidant assays rapidly assessed and contrasted antioxidant activity or the free radical scavenging activity of the main polyphenolic classes in MMEs, and the antioxidant capacity was mostly affected by the presence of gallic acid. Thus, this study establishes a powerful and meaningful approach for MME quality control and for assessing in vitro antioxidant activity.
通过将高效液相色谱二极管阵列检测器（HPLC-DAD）方法与高效薄层色谱（HPTLC）相结合，开发了一种用于传统药物马俊木帕希依拉（MME）化学指纹图谱分析和定量分析的方法，并使用HPLC与超高效液相色谱-二极管阵列检测器-四极杆-轨道阱质谱联用方法鉴定了其成分化合物。此外，基于成分清除自由基的能力，采用体外方法评估了MME的抗氧化能力。使用带有HPTLC的HPLC-DAD方法能够轻松验证化学指纹图谱结果，并对市售MME中的三种特征成分，即没食子酸（1）、大豆苷元（2）和淫羊藿苷（3）进行定量。这三种化合物在测试范围内呈现出优异的回归值（=0.9999），方法回收率在100.49%至100.68%之间。指纹图谱有27个共同特征峰，其中13个通过快速超高效液相色谱-二极管阵列检测器-四极杆-轨道阱质谱分析得到验证。体外抗氧化试验快速评估并对比了MME中主要多酚类物质的抗氧化活性或自由基清除活性，且抗氧化能力主要受没食子酸的影响。因此，本研究为MME的质量控制和体外抗氧化活性评估建立了一种强大且有意义的方法。
