马弥然乳膏对特应性皮炎样皮肤损伤的体内抑制作用。

The Suppressive Effect of Mamiran Cream on Atopic Dermatitis-Like Skin Lesions In Vivo.

作者信息

Aierken Kailibinuer, Luo Yuqing, Maiwulanjiang Maitinuer, Wu Tao, Aisa H A

机构信息

State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China.

University of the Chinese Academy of Sciences, Beijing 100039, China.

出版信息

Evid Based Complement Alternat Med. 2021 Nov 30;2021:2854238. doi: 10.1155/2021/2854238. eCollection 2021.

BACKGROUND

The Chinese herbal formula Mamiran cream (MMC) has been known for its ameliorative effects on diverse skin diseases, such as eczema. Atopic dermatitis (AD; eczema) is a chronic recurrent skin disease dominated by T-helper type 2-driven inflammation (Th2).

OBJECTIVE

In this study, the inhibitory effect of MMC on AD was investigated in vivo.

METHODS

An animal model was established by sensitization with 2,4-dinitrochlorobenzene (DNCB) on the skin of SD rats. Cutaneous administration of MMC was applied, and its mechanism of action was investigated via RT-PCR and IHC assay.

RESULT

Our data showed that topical application of MMC reduced the skin severity scores and alleviated the histological changes. Furthermore, immunohistochemical analysis demonstrated that MMC significantly decreased the levels of Th2 cytokine IL-5 and IL-4Ra in the skin lesion. In addition, it was demonstrated that MMC downregulated the mRNA expression of TNF-, IL-1, IL-6, IL-10, and TLR4. Moreover, MMC inhibited the activation of NF-B, JNK1, and STAT6 pathways in skin lesions.

CONCLUSIONS

Our findings suggest that MMC exhibits the inhibitory effect on AD, suggesting that MMC may be a potential therapeutic agent for this atopic disorder.

背景

中药配方马弥然乳膏（MMC）因其对多种皮肤病如湿疹具有改善作用而闻名。特应性皮炎（AD；湿疹）是一种以2型辅助性T细胞驱动的炎症（Th2）为主导的慢性复发性皮肤病。

目的

本研究在体内研究MMC对AD的抑制作用。

方法

通过在SD大鼠皮肤上用2,4-二硝基氯苯（DNCB）致敏建立动物模型。应用MMC进行皮肤给药，并通过逆转录-聚合酶链反应（RT-PCR）和免疫组化分析研究其作用机制。

结果

我们的数据表明，局部应用MMC可降低皮肤严重程度评分并减轻组织学变化。此外，免疫组化分析表明，MMC可显著降低皮肤病变中Th2细胞因子白细胞介素-5（IL-5）和白细胞介素-4受体α（IL-4Ra）的水平。此外，还证明MMC可下调肿瘤坏死因子-α（TNF-α）、白细胞介素-1（IL-1）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）和Toll样受体4（TLR4）的mRNA表达。此外，MMC可抑制皮肤病变中核因子-κB（NF-κB）、应激活化蛋白激酶1（JNK1）和信号转导子和转录激活子6（STAT6）通路的激活。