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解析基质硬度在利用基于脂质的纳米载体增强干细胞中质粒 DNA 内化效率中的作用。

Deciphering the role of substrate stiffness in enhancing the internalization efficiency of plasmid DNA in stem cells using lipid-based nanocarriers.

机构信息

BioIntel Research Laboratory, Department of Chemical and Petroleum Engineering, Bioengineering Graduate Program, School of Engineering, University of Kansas, Lawrence, KS, USA.

出版信息

Nanoscale. 2018 May 17;10(19):8947-8952. doi: 10.1039/c8nr01516c.

DOI:10.1039/c8nr01516c
PMID:29693099
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5957767/
Abstract

This study investigates the role of substrate stiffness in the non-viral transfection of human adipose-derived stem cells (hASCs) with the aim to maximize the hASC expression of vascular endothelial growth factor (VEGF). The results confirm the direct effect of substrate stiffness in regulating cytoskeletal remodeling and corresponding plasmid internalization.

摘要

本研究旨在探讨基质硬度在非病毒转染人脂肪来源干细胞（hASCs）以最大程度提高血管内皮生长因子（VEGF）表达中的作用。结果证实了基质硬度在调节细胞骨架重塑和相应质粒内化方面的直接作用。

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Sci Rep. 2017 Jul 26;7(1):6577. doi: 10.1038/s41598-017-06028-y.
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Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration.开发用于组织修复和再生的内源性干细胞招募生物活性材料的策略。
Adv Drug Deliv Rev. 2017 Oct 1;120:50-70. doi: 10.1016/j.addr.2017.07.011. Epub 2017 Jul 19.
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Nanodiamond-based injectable hydrogel for sustained growth factor release: Preparation, characterization and in vitro analysis.
用于神经损伤治疗的胶质细胞中的RNA干扰
J Tissue Eng. 2020 Jul 6;11:2041731420939224. doi: 10.1177/2041731420939224. eCollection 2020 Jan-Dec.
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Nucleic acid delivery to mesenchymal stem cells: a review of nonviral methods and applications.核酸递送至间充质干细胞：非病毒方法及应用综述
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Biomaterial substrate modifications that influence cell-material interactions to prime cellular responses to nonviral gene delivery.生物材料基底修饰影响细胞与材料的相互作用，从而启动细胞对非病毒基因传递的反应。
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Mesenchymal stromal cells regulate the cell mobility and the immune response during osteogenesis through secretion of vascular endothelial growth factor A.间充质基质细胞通过分泌血管内皮生长因子 A 调节成骨过程中的细胞迁移和免疫反应。
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