解析基质硬度在利用基于脂质的纳米载体增强干细胞中质粒 DNA 内化效率中的作用。
Deciphering the role of substrate stiffness in enhancing the internalization efficiency of plasmid DNA in stem cells using lipid-based nanocarriers.
机构信息
BioIntel Research Laboratory, Department of Chemical and Petroleum Engineering, Bioengineering Graduate Program, School of Engineering, University of Kansas, Lawrence, KS, USA.
出版信息
Nanoscale. 2018 May 17;10(19):8947-8952. doi: 10.1039/c8nr01516c.
Abstract
This study investigates the role of substrate stiffness in the non-viral transfection of human adipose-derived stem cells (hASCs) with the aim to maximize the hASC expression of vascular endothelial growth factor (VEGF). The results confirm the direct effect of substrate stiffness in regulating cytoskeletal remodeling and corresponding plasmid internalization.
摘要
本研究旨在探讨基质硬度在非病毒转染人脂肪来源干细胞（hASCs）以最大程度提高血管内皮生长因子（VEGF）表达中的作用。结果证实了基质硬度在调节细胞骨架重塑和相应质粒内化方面的直接作用。
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Stem Cell Res Ther. 2017 Apr 26;8(1):94. doi: 10.1186/s13287-017-0558-6.
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