Stemedica Inc., San Diego, CA 92121, USA.
Department of Physics, University of California-San Diego, La Jolla, CA 92093, USA.
Sci Rep. 2016 Sep 21;6:33411. doi: 10.1038/srep33411.
The dependences of spreading and differentiation of stem cells plated on hydrogel and silicone gel substrates on the rigidity and porosity of the substrates have recently been a subject of some controversy. In experiments on human mesenchymal stem cells plated on soft, medium rigidity, and hard silicone gels we show that harder gels are more osteogenic, softer gels are more adipogenic, and cell spreading areas increase with the silicone gel substrate rigidity. The results of our study indicate that substrate rigidity induces some universal cellular responses independently of the porosity or topography of the substrate.
最近，细胞在水凝胶和硅凝胶基质上的铺展和分化的依赖性与基质的硬度和孔隙率有关，这一问题存在一些争议。在对铺展在柔软、中等硬度和硬硅凝胶上的人骨髓间充质干细胞的实验中，我们发现，较硬的凝胶更具成骨性，较软的凝胶更具成脂性，且细胞铺展面积随硅凝胶基质硬度的增加而增加。我们的研究结果表明，基质硬度会引起一些普遍的细胞反应，而与基质的孔隙率或形貌无关。
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