College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China.
J Cell Physiol. 2018 Oct;233(10):6965-6974. doi: 10.1002/jcp.26614. Epub 2018 Apr 25.
Circular RNAs (circRNAs) in various tissues and cell types from mammalian sources have been studied. However, present knowledge on circRNAs in the development of pre-receptive endometrium (PE) in dairy goats is limited. In the pre-receptive endometrium of dairy goats, higher circRNA3175 (ciR3175) levels, lower miR-182 levels and higher Testin (TES) levels were detected. And ciR3175 could decreased the miR-182 levels by acting as a miRNA sponge, and miR-182 could down-regulated the expression level of TES via the predicted target site in endometrial epithelial cells (EECs) in vitro. Via this way, ciR3175 functioned as a competing endogenous RNAs (ceRNA) that sequestered miR-182, thereby protecting TES transcripts from miR-182-mediated suppression in EECs in vitro. Further, TES inhibited EECs apoptosis by decreasing the expression level of BCL-2/BAX via the MAPK pathway. Thus, a ciR3175-miR182-TES pathway in the endometrium was identified in EECs, and the modulation of which could emerge as a potential target in regulating the pre-receptive endometrium development in dairy goats.
环状 RNA（circRNAs）已在哺乳动物来源的各种组织和细胞类型中进行了研究。然而，目前关于奶牛山羊接受前子宫内膜（PE）发育过程中 circRNAs 的知识有限。在奶牛山羊的接受前子宫内膜中，检测到更高的 circRNA3175（ciR3175）水平、更低的 miR-182 水平和更高的 Testin（TES）水平。ciR3175 可以通过充当 miRNA 海绵来降低 miR-182 水平，并且 miR-182 可以通过预测的靶位点在体外的子宫内膜上皮细胞（EECs）中下调 TES 的表达水平。通过这种方式，ciR3175 作为一种竞争性内源 RNA（ceRNA）起作用，可通过与 miR-182 结合来保护 TES 转录物免受 miR-182 在体外 EECs 中的抑制作用。此外，TES 通过 MAPK 通路降低 BCL-2/BAX 的表达水平来抑制 EECs 凋亡。因此，在 EECs 中鉴定出子宫内膜中的 ciR3175-miR182-TES 通路，其调控可能成为调节奶牛山羊接受前子宫内膜发育的潜在靶点。
