a Department of Human Biology , NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre+ , ER Maastricht , The Netherlands.
b Top Institute Food and Nutrition , PA Wageningen , the Netherlands.
Adipocyte. 2018;7(2):106-112. doi: 10.1080/21623945.2018.1464366. Epub 2018 Apr 25.
The intestinal microbiota may contribute to the development of obesity by affecting host lipid metabolism and insulin sensitivity. To investigate the effects of microbiota manipulation on ex vivo basal and β-adrenergically-stimulated lipolysis in human adipocytes, 36 obese men were randomized to amoxicillin (broad-spectrum antibiotic), vancomycin (narrow-spectrum antibiotic) or placebo treatment (7 d, 1500 mg/d). Before and after treatment, ex vivo adipose tissue lipolysis was assessed under basal conditions and during stimulation with the non-selective β-agonist isoprenaline using freshly isolated mature adipocytes. Gene (targeted microarray) and protein expression were analyzed to investigate underlying pathways. Antibiotics treatment did not significantly affect basal and maximal isoprenaline-mediated glycerol release from adipocytes. Adipose tissue β-adrenoceptor expression or post-receptor signalling was also not different between groups. In conclusion, 7 d oral antibiotics treatment has no effect on ex vivo lipolysis in mature adipocytes derived from adipose tissue of obese insulin resistant men.
肠道微生物群可能通过影响宿主脂质代谢和胰岛素敏感性而导致肥胖的发生。为了研究微生物群操作对人体脂肪细胞体外基础和β-肾上腺素能刺激脂肪分解的影响，36 名肥胖男性被随机分为阿莫西林（广谱抗生素）、万古霉素（窄谱抗生素）或安慰剂治疗组（7 天，1500mg/d）。在治疗前后，使用新鲜分离的成熟脂肪细胞，在基础条件下以及用非选择性β-激动剂异丙肾上腺素刺激下，评估体外脂肪组织脂肪分解。分析了基因（靶向微阵列）和蛋白质表达，以研究潜在途径。抗生素治疗并未显著影响脂肪细胞的基础和最大异丙肾上腺素介导的甘油释放。脂肪组织β-肾上腺素受体表达或受体后信号转导在各组之间也没有差异。总之，7 天口服抗生素治疗对肥胖、胰岛素抵抗男性脂肪组织来源的成熟脂肪细胞的体外脂肪分解没有影响。
