College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
Department of Preventive Medicine and Public Health, Faculty of Veterinary Science, University of Nyala, P.O. Box, 155 Nyala, Sudan.
Int J Mol Sci. 2018 Apr 25;19(5):1291. doi: 10.3390/ijms19051291.
To investigate the roles and explore the altered expression of microRNAs (miRNAs) and mRNAs in chicken embryos in response to Newcastle disease virus (NDV) infection, deep sequencing was performed. Then, a conjoint analysis of small RNA-seq and mRNA-seq was performed to screen interactional miRNA⁻mRNA pairs during NDV infection. In total, 15 and 17 up- and downregulated miRNAs were identified that potentially targeted 4279 and 6080 mRNAs in NDV-infected chicken embryonic tissues, respectively; in addition, 595 upregulated and 480 downregulated mRNAs were identified. The conjoint analysis of the obtained data identified 1069 miRNA⁻mRNA pairs. Among these pairs, 130 pairs were related to immune or inflammatory responses. The relationship between gga-miR-203a and its target transglutaminase 2 (TGM2) was confirmed using a dual-luciferase reporter system and a real time quantitative polymerase chain reaction (RT-qPCR) assay. Overall, the discovery of miRNAs, mRNAs, and their potential pairing relationships, which may be involved in the regulation of NDV infection, will facilitate our understanding of the complex regulatory relationship between the host and the virus.
为了研究微 RNA（miRNA）和 mRNAs 在鸡胚中对新城疫病毒（NDV）感染的反应及其作用，进行了深度测序。然后，对小 RNA-seq 和 mRNA-seq 进行联合分析，以筛选 NDV 感染过程中相互作用的 miRNA⁻mRNA 对。共鉴定出 15 个上调和 17 个下调的 miRNA，分别可能靶向 NDV 感染鸡胚组织中的 4279 和 6080 个 mRNAs；此外，还鉴定出 595 个上调和 480 个下调的 mRNAs。对获得的数据进行联合分析，鉴定出 1069 个 miRNA⁻mRNA 对。其中，130 对与免疫或炎症反应有关。利用双荧光素酶报告系统和实时定量聚合酶链反应（RT-qPCR）实验证实了gga-miR-203a 与其靶基因转谷氨酰胺酶 2（TGM2）之间的关系。总之，miRNA、mRNAs 及其潜在配对关系的发现，可能参与了 NDV 感染的调控，将有助于我们理解宿主与病毒之间复杂的调控关系。
Zotero 插件
