Chen Junjun, Du Guanhuan, Wang Yufeng, Shi Linjun, Mi Jun, Tang Guoyao
Department of Oral Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Biochemistry and Molecular Cell Biology, Shanghai Key Laboratory of Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Department of Oral Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2017 Oct;124(4):390-402.e17. doi: 10.1016/j.oooo.2017.05.513. Epub 2017 Jun 8.
Oral lichen planus (OLP), a chronic inflammatory disease of unknown etiology, is considered a potentially malignant oral disorder. The aim of the present study was to analyze candidate microRNAs (miRNAs) and genes from patients with OLP and healthy controls (HCs).
Biopsy specimens of the oral mucosa were collected from patients with OLP (n = 9) and from HCs (n = 4). Differentially expressed miRNAs and differentially expressed genes were screened by using next-generation sequencing with DESeq and edgeR software algorithms.
A total of 94 differentially expressed miRNAs and 599 differentially expressed genes were detected in OLP. Potential regulatory miRNAs and genes were obtained by analyzing miRNA-messenger RNA networks. Of these, 5 downregulated miRNAs-Hsa-miR-135 a-5 p (P = .33), hsa-miR-128-3 p (P = .03), hsa-miR-218-5 p (P = .01), hsa-miR-125 a-5 p (P = .01), and hsa-let-7 e-5 p (P = .04)-were the most promising biomarkers in patients with OLP compared with HCs. The identified differentially expressed genes were significantly enriched in "inflammatory" events and immune-related terms through Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analysis.
The integrative analysis of messenger RNA and miRNA profiles provides important information to elucidate gene expression mechanisms and a comprehensive perspective to study the etiology and pathogenesis of OLP.
口腔扁平苔藓（OLP）是一种病因不明的慢性炎症性疾病，被认为是一种潜在的恶性口腔疾病。本研究的目的是分析OLP患者和健康对照者（HCs）的候选微小RNA（miRNA）和基因。
从OLP患者（n = 9）和HCs（n = 4）中收集口腔黏膜活检标本。使用DESeq和edgeR软件算法通过下一代测序筛选差异表达的miRNA和差异表达的基因。
在OLP中检测到总共94种差异表达的miRNA和599种差异表达的基因。通过分析miRNA-信使RNA网络获得潜在的调控miRNA和基因。其中，与HCs相比，5种下调的miRNA——Hsa-miR-135 a-5 p（P = 0.33）、hsa-miR-128-3 p（P = 0.03）、hsa-miR-218-5 p（P = 0.01）、hsa-miR-125 a-5 p（P = 0.01）和hsa-let-7 e-5 p（P = 0.04）——是OLP患者中最有前景的生物标志物。通过京都基因与基因组百科全书和基因本体分析，鉴定出的差异表达基因在“炎症”事件和免疫相关术语中显著富集。
信使RNA和miRNA谱的综合分析为阐明基因表达机制提供了重要信息，并为研究OLP的病因和发病机制提供了全面的视角。
