Department of Biochemistry and Biotechnology, University of Thessaly, 41500 Larissa, Greece.
Laboratory of Clinical Virology, University of Crete, Medical School, 71409 Heraklion, Crete, Greece.
Mol Med Rep. 2018 Jun;17(6):8325-8331. doi: 10.3892/mmr.2018.8924. Epub 2018 Apr 24.
Coffee is a popular beverage that contains various bioactive compounds. However, its molecular mechanism of action is not fully elucidated. In this context, two previously characterized coffee extracts, a lightly roasted and the corresponding green one, were investigated for their effect on nuclear factor erythroid 2‑related factor 2 (Nrf2) target gene expression in myoblasts and endothelial cells using quantitative PCR. The tested concentrations were non‑cytotoxic and led to improved redox cell status, as was evident by increased reduced glutathione (GSH) levels. In both cell lines, the roasted extract upregulated gene expression more readily than its green counterpart leading to increased NAD(P)H quinone dehydrogenase 1 and γ‑glutamyl cysteine ligase catalytic subunit, among others. The green extract had a mixed effect on the endothelial cells, while, as regards the myoblasts it caused the downregulation of some Nrf‑target genes. Therefore, a potential dose‑ and roasting‑dependent mechanism is proposed in the current study, accounting for coffee's antioxidant activity.
咖啡是一种受欢迎的饮料，含有各种生物活性化合物。然而，其分子作用机制尚未完全阐明。在这种情况下，使用定量 PCR 研究了两种先前表征的咖啡提取物（轻度烘焙和相应的绿色提取物）对成肌细胞和内皮细胞中核因子红细胞 2 相关因子 2（Nrf2）靶基因表达的影响。测试浓度是非细胞毒性的，并导致氧化还原细胞状态得到改善，这表现在还原型谷胱甘肽 (GSH) 水平增加。在两种细胞系中，烘焙提取物比其绿色对应物更容易上调基因表达，导致 NAD(P)H 醌脱氢酶 1 和 γ-谷氨酰半胱氨酸连接酶催化亚基等基因的表达增加。绿色提取物对内皮细胞有混合作用，而对于成肌细胞，它导致一些 Nrf-靶基因的下调。因此，本研究提出了一种潜在的剂量和烘焙依赖机制，解释了咖啡的抗氧化活性。
