Kheirbakhsh Raheleh, Haddadi Mahnaz, Muhammadnejad Ahad, Abdollahi Alireza, Shahi Farshad, Amanpour-Gharaei Behzad, Abrahim-Habibi Azadeh, Barati Tahereh, Amanpour Saeid
Cancer Biology Research Center, Cancer research Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran,
Vali-e-Asr Reproductive Health research center, Tehran University of Medical Sciences, Tehran, Iran.
Acta Neurobiol Exp (Wars). 2018;78(1):51-59.
Alzheimer's disease (AD) is a mental impairment and neural degeneration which causes progressive loss of memory and cognitive functions. This age‑dependent illness is associated with extracellular amyloid plaques accumulation and twisted neurofibrillary tangles. Amyloid plaques are experimentally generated in animal models in order to investigate the disease process. In this study, we followed a rat model of AD for over a year. Wistar rats were divided randomly into two groups as control group (surgery without injection Aβ), and experimental group (two‑sided intrahippocampal amyloid‑beta injection into hippocampus). From each group, three animals were investigated 42 days after injection, and the remaining four animals were studied after one year. All animals were tested for learning abilities and memory. Finally, samples from blood, brain, heart, kidney, liver, colon and spleen were examined. In the experimental group, the size of amyloid plaques were increased significantly after one year \r\nand learning abilities and memory were concomitantly decreased. Onsets of various other conditions such as liver and kidney disorders, diabetes, and metabolic syndrome were observed, which indicates that the animals may be prone to cardiovascular disorders and ischemia.
阿尔茨海默病（AD）是一种精神障碍和神经退行性疾病，会导致记忆力和认知功能逐渐丧失。这种与年龄相关的疾病与细胞外淀粉样斑块的积累和扭曲的神经原纤维缠结有关。为了研究疾病过程，在动物模型中通过实验生成淀粉样斑块。在本研究中，我们对AD大鼠模型进行了一年多的跟踪观察。将Wistar大鼠随机分为两组，即对照组（手术但不注射Aβ）和实验组（双侧海马内注射β淀粉样蛋白）。注射后42天，每组选取3只动物进行研究，其余4只动物在一年后进行研究。对所有动物进行学习能力和记忆力测试。最后，对血液、大脑、心脏、肾脏、肝脏、结肠和脾脏的样本进行检查。在实验组中，一年后淀粉样斑块的大小显著增加，同时学习能力和记忆力下降。还观察到了其他各种病症的发作，如肝脏和肾脏疾病、糖尿病和代谢综合征，这表明这些动物可能易患心血管疾病和局部缺血。
