Borah Sudarshana, Sarkar Priyanka, Sharma Hemanta Kumar
Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam 786004 India.
DBT Wellcome Lab, Asian Healthcare Foundation, Asian Institute of Gastroenterology, Hyderabad, India.
3 Biotech. 2021 May;11(5):240. doi: 10.1007/s13205-021-02793-w. Epub 2021 Apr 26.
Scientific pieces of evidence support the pharmacological activity of for its antidepressant, analgesic, anticonvulsant and antioxidant effect. Here, we evaluate the bioactivity of essential oil and the various polarity-based solvent partitioned fractions obtained from for anti-amnesia, anxiolytic and antidepressant activities using Elevated plus maze and Morris water maze models. The cold maceration technique using methanol was adopted for extraction from dried powdered rhizomes and essential oil was extracted by hydrodistillation method. Partitioning of the methanolic extract based on solvent polarity by hexane, ethyl acetate, and methanol was continued, followed by column chromatography of the ethyl acetate fraction. Suspensions were prepared for fractions (dissolved in distilled water) and essential oil (dissolved in tween 20) at 200 mg/kg and 400 mg/kg after acute toxicity study and were orally administered to Wistar albino female rats after the orientation of hypoxia by sodium nitrite (50 mg/kg) and amnesia by scopolamine (1 mg/kg). Behavioural observations, biochemical and histopathological examinations were carried out for all the treated groups. Diazepam (12 mg/kg) and galantamine (3 mg/kg) were used as standard drugs for this study against hypoxia and amnesia. Data acquired from behavioural, biochemical (acetylcholinesterase, myeloperoxidase, superoxide dismutase, reduced glutathione, catalase) and histopathological studies have illustrated that fraction II acquires highly significant memory-enhancing, anxiolytic and antidepressant effects. Rest fractions (I and III) and essential oil showed moderate efficacy. In prospects, identification of active molecules from the most active fraction (fraction II) and further studies on a molecular basis would substantiate its specific mechanism of neuroprotective action.
科学证据支持其具有抗抑郁、镇痛、抗惊厥和抗氧化作用的药理活性。在此，我们使用高架十字迷宫和莫里斯水迷宫模型评估了从[植物名称未给出]中获得的精油以及各种基于极性的溶剂分配馏分的抗遗忘、抗焦虑和抗抑郁活性。采用甲醇冷浸法从干燥的根茎粉末中提取，并用蒸馏法提取精油。继续用己烷、乙酸乙酯和甲醇根据溶剂极性对甲醇提取物进行分配，然后对乙酸乙酯馏分进行柱色谱分离。在急性毒性研究后，将馏分（溶于蒸馏水）和精油（溶于吐温20）分别配制成200mg/kg和400mg/kg的悬浮液，在通过亚硝酸钠（50mg/kg）诱导缺氧和东莨菪碱（1mg/kg）诱导失忆后，口服给予Wistar白化雌性大鼠。对所有处理组进行行为观察、生化和组织病理学检查。地西泮（12mg/kg）和加兰他敏（3mg/kg）用作本研究中抗缺氧和抗失忆的标准药物。从行为、生化（乙酰胆碱酯酶、髓过氧化物酶、超氧化物歧化酶、还原型谷胱甘肽、过氧化氢酶）和组织病理学研究中获得的数据表明，馏分II具有高度显著的记忆增强、抗焦虑和抗抑郁作用。其余馏分（I和III）以及精油显示出中等疗效。展望未来，从活性最高的馏分（馏分II）中鉴定活性分子并在分子基础上进行进一步研究，将证实其神经保护作用的具体机制。
