Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, China; Beijing Advanced Innovation Centre for Biomedical Engineering, Beihang University, Beijing, China.
Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, China; Beijing Advanced Innovation Centre for Biomedical Engineering, Beihang University, Beijing, China.
Biophys J. 2018 Apr 24;114(8):1988-2000. doi: 10.1016/j.bpj.2018.02.041.
The periodontal ligament (PDL) is highly ordered connective tissue located between the alveolar bone and cementum. An aligned and organized architecture is required for its physiological function. We applied micropatterning technology to arrange PDL cells in 10- or 20-μm-wide extracellular protein patterns. Cell and nuclear morphology, cytoskeleton, proliferation, differentiation, and matrix metalloproteinase system expression were investigated. Micropatterning clearly elongated PDL cells with a low cell-shape index and low spreading area. The nucleus was also elongated as nuclear height increased, but the nuclear volume remained intact. The cytoskeleton was rearranged to form prominent bundles at cells' peripheral regions. Moreover, proliferation was promoted by 10- and 20-μm micropatterning. Osteogenesis and adipogenesis were each inhibited, but micropatterning increased PDL cells' stem cell markers. β-catenin was expelled to cytoplasm. YAP/TAZ nuclear localization and activity both decreased, which might indicate their role in micropatterning-regulated differentiation. Collagen Ι expression increased in micropatterned groups. It might be due to the decreased expression of matrix metalloproteinase-1, 2 and the tissue inhibitor of metalloproteinase-1 gene expression elevation in micropatterned groups. The findings of this study provide insight into the effects of a micropatterned surface on PDL cell behavior and may be applicable in periodontal tissue regeneration.
牙周韧带（PDL）是一种高度有序的结缔组织，位于牙槽骨和牙骨质之间。为了实现其生理功能，需要有一个排列整齐、组织有序的结构。我们应用微图案化技术，将 PDL 细胞排列在 10 或 20μm 宽的细胞外蛋白图案中。研究了细胞和核形态、细胞骨架、增殖、分化和基质金属蛋白酶系统的表达。微图案化明显拉长了 PDL 细胞，细胞形态指数和铺展面积较低。核也随着核高度的增加而拉长，但核体积保持不变。细胞骨架重新排列，在细胞的外周区域形成明显的束。此外，10μm 和 20μm 的微图案化促进了细胞的增殖。成骨和脂肪形成均受到抑制，但微图案化增加了 PDL 细胞的干细胞标志物。β-连环蛋白被挤出细胞质。YAP/TAZ 的核定位和活性均降低，这可能表明它们在微图案化调节分化中的作用。胶原 Ι 在微图案化组中的表达增加。这可能是由于微图案化组中基质金属蛋白酶-1、2 的表达降低，以及组织金属蛋白酶抑制剂-1 基因表达升高所致。本研究结果深入了解了微图案化表面对 PDL 细胞行为的影响，可能适用于牙周组织再生。
