Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, Tennessee 38163, United States.
Department of Orthopaedic Surgery and Biomedical Engineering, College of Medicine, University of Tennessee Health Science Center, Memphis, Tennessee 38163, United States.
ACS Appl Mater Interfaces. 2024 Nov 6;16(44):59979-59990. doi: 10.1021/acsami.4c13830. Epub 2024 Oct 28.
Periodontitis and severe trauma are major causes of damage to the periodontal ligament (PDL). Repairing the native conditions of the PDL is essential for the stability of the tissue and its interfaces. Bioprinting periodontal ligament stem cells (PDLSCs) is an interesting approach to guide the regeneration of PDL and interfacial integration. Herein, a collagen-based bioink mimicking the native extracellular matrix conditions and carrying PDLSCs was tested to guide the periodontal ligament organization. The bioink was tested at two different concentrations (10 and 15 mg/mL) and characterized by swelling and degradation, microstructural organization, and rheological properties. The biological properties were assessed after loading PDLSCs into bioinks for bioprinting. The characterization was performed through cell viability, alizarin red assay, and expression for , , , and . The in vivo biocompatibility of the PDLSC-laden bioinks was verified using subcutaneous implantation in mice. Later, the ability of the bioprinted PDLSC-laden bioinks on dental root fragments to form PDL was also investigated in mice for 4 and 10 weeks. The bioinks demonstrated typical shear-thinning behavior, a porous microstructure, and stable swelling and degradation characteristics. Both concentrations were printable and provided suitable conditions for a high cell survival, proliferation, and differentiation. PDLSC-laden bioinks demonstrated biocompatibility , and the bioprinted scaffolds on the root surface evidenced PDLSC alignment, organization, and PDLSC migration to the root surface. The versatility of collagen-based bioinks provides native ECM conditions for PDLSC proliferation, alignment, organization, and differentiation, with translational applications in bioprinting scaffolds for PDL regeneration.
牙周炎和严重创伤是牙周韧带（PDL）损伤的主要原因。修复 PDL 的原生条件对于组织及其界面的稳定性至关重要。生物打印牙周韧带干细胞（PDLSCs）是一种很有前途的方法，可以指导 PDL 的再生和界面整合。在此，测试了一种模仿天然细胞外基质条件并携带 PDLSCs 的基于胶原蛋白的生物墨水，以指导牙周韧带组织的形成。该生物墨水在两种不同浓度（10 和 15mg/mL）下进行了测试，并通过溶胀和降解、微观结构组织和流变性能进行了表征。在将 PDLSCs 加载到生物墨水中进行生物打印后，评估了其生物学特性。通过细胞活力、茜素红测定和基因表达（、、、和）评估了生物墨水的生物特性。通过皮下植入小鼠来验证 PDLSC 负载生物墨水的体内生物相容性。随后，还研究了生物打印的 PDLSC 负载生物墨水在牙齿根部碎片上形成 PDL 的能力，在小鼠体内进行了 4 周和 10 周的研究。生物墨水表现出典型的剪切变稀行为、多孔微观结构以及稳定的溶胀和降解特性。两种浓度均可打印，并为高细胞存活率、增殖和分化提供了合适的条件。PDLSC 负载的生物墨水具有生物相容性，在牙根表面打印的支架上证明了 PDLSC 的排列、组织和 PDLSC 向牙根表面的迁移。基于胶原蛋白的生物墨水的多功能性为 PDLSC 的增殖、排列、组织和分化提供了原生 ECM 条件，在 PDL 再生的生物打印支架中有转化应用的潜力。
