Barkhordar R A, Desouza Y G
Department of Restorative Dentistry and Stomatology, University of California School of Dentistry, San Francisco.
Oral Surg Oral Med Oral Pathol. 1988 Jun;65(6):763-6. doi: 10.1016/0030-4220(88)90026-6.
Both B-lymphocyte-and T-lymphocyte-mediated immunologic reactions have been implicated in the pathogenesis of human periapical lesions. The purpose of this study was to identify various subpopulations of T-lymphocytes in these lesions. Fifteen periapical lesions were obtained during periapical surgery. Each specimen was quick-frozen and stored in liquid nitrogen. Immunoperoxidase staining for the presence of T-lymphocyte subpopulation, was done with the use of the monoclonal antibody and Vecta-stain ABC/peroxidase kit. Cryostat tissue sections were incubated with 3% normal horse serum. Specific monoclonal antibody (Coulter T4, T8, T11) was placed on the section, washed, and then covered with biotinylated antibody to mouse IgG. After TRIS HC1 washing, the ABC/peroxidase solution was placed on the section and 3,3-diaminobenzidine was applied; the section was washed and then counterstained with 2% methyl green. After dehydration, sections were mounted and examined under the light microscope. The presence of lymphocytes was confirmed by the appearance of brown rings around cells with lymphocyte morphology. Fourteen lesions were diagnosed as granuloma and stained positively for pan T-lymphocytes (T11), T helper cells (T4), and T cytotoxic cells (T8). The remaining specimen, diagnosed as an apical scar, contained no T-lymphocytes. The presence of T-cells in periapical lesions indicates that cell-mediated reactions participate in the pathogenesis of these lesions.
B淋巴细胞介导的免疫反应和T淋巴细胞介导的免疫反应均被认为与人类根尖周病变的发病机制有关。本研究的目的是确定这些病变中T淋巴细胞的各种亚群。在根尖周手术中获取了15个根尖周病变组织。每个标本迅速冷冻并保存在液氮中。使用单克隆抗体和Vecta-stain ABC/过氧化物酶试剂盒对T淋巴细胞亚群进行免疫过氧化物酶染色。将低温恒温器组织切片与3%正常马血清孵育。将特异性单克隆抗体（库尔特T4、T8、T11）置于切片上，冲洗后，再覆盖生物素化的抗小鼠IgG抗体。经TRIS HC1冲洗后，将ABC/过氧化物酶溶液置于切片上，并加入3,3-二氨基联苯胺；冲洗切片，然后用2%甲基绿复染。脱水后，将切片封固并在光学显微镜下检查。通过淋巴细胞形态的细胞周围出现棕色环来确认淋巴细胞的存在。14个病变被诊断为肉芽肿，对全T淋巴细胞（T11）、T辅助细胞（T4）和T细胞毒性细胞（T8）呈阳性染色。其余标本被诊断为根尖瘢痕，未含有T淋巴细胞。根尖周病变中T细胞的存在表明细胞介导的反应参与了这些病变的发病机制。
