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用回收制备液相色谱法从紫甘蓝中分离高纯度花色苷单体及其光稳定性。

Isolation of High Purity Anthocyanin Monomers from Red Cabbage with Recycling Preparative Liquid Chromatography and Their Photostability.

机构信息

Xinjiang Production & Construction Group, Key Laboratory of Biological Resource Protection and Utilization of Tarim Basin, Alar 843300, China.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.

出版信息

Molecules. 2018 Apr 24;23(5):991. doi: 10.3390/molecules23050991.

DOI:10.3390/molecules23050991
PMID:29695065
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6099797/
Abstract

Anthocyanins from red cabbage are of great importance for their applications in the food industry as natural colorants and their beneficial effects on human wellness as natural antioxidants. This study aimed to develop an effective method for the isolation of anthocyanins with the help of a combination of alternate recycling and direct recycling preparative liquid chromatography. Ten major components of anthocyanins from red cabbage were isolated and their structures were identified by HPLC-MS/MS. Meanwhile, the stability of the isolated anthocyanins under various light conditions was also investigated so as to provide data for their storage. In sum, the results showed that twin column recycling preparative chromatography is an effective method for the isolation of anthocyanin monomers with similar structures. Besides, the stability of various anthocyanins from red cabbage was related to the number of acylated groups and mainly affected by illumination.

摘要

红甘蓝中的花色苷因其在食品工业中作为天然色素的应用和作为天然抗氧化剂对人类健康的有益作用而非常重要。本研究旨在借助交替循环和直接循环制备液相色谱的组合开发一种有效的花色苷分离方法。从红甘蓝中分离出 10 种主要花色苷成分，并通过 HPLC-MS/MS 鉴定其结构。同时，还研究了分离出的花色苷在各种光照条件下的稳定性，为其储存提供数据。总之，结果表明，双柱循环制备色谱是分离结构相似的花色苷单体的有效方法。此外，红甘蓝中各种花色苷的稳定性与酰化基团的数量有关，主要受光照影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2322/6099797/89eb7c75ea1d/molecules-23-00991-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2322/6099797/327387a0dc0a/molecules-23-00991-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2322/6099797/c129a6f8dc11/molecules-23-00991-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2322/6099797/a34b1d7a8274/molecules-23-00991-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2322/6099797/76189185c775/molecules-23-00991-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2322/6099797/4ebd19f15b45/molecules-23-00991-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2322/6099797/103360cf720e/molecules-23-00991-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2322/6099797/89eb7c75ea1d/molecules-23-00991-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2322/6099797/327387a0dc0a/molecules-23-00991-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2322/6099797/c129a6f8dc11/molecules-23-00991-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2322/6099797/a34b1d7a8274/molecules-23-00991-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2322/6099797/76189185c775/molecules-23-00991-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2322/6099797/4ebd19f15b45/molecules-23-00991-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2322/6099797/103360cf720e/molecules-23-00991-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2322/6099797/89eb7c75ea1d/molecules-23-00991-g007.jpg

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