从未致敏小鼠中激活并扩增半抗原特异性和蛋白质特异性辅助性T细胞。
Activation and expansion of hapten- and protein-specific T helper cells from nonsensitized mice.
作者信息
Hauser C, Katz S I
机构信息
Dermatology Branch, National Cancer Institute, Bethesda, MD 20892.
出版信息
Proc Natl Acad Sci U S A. 1988 Aug;85(15):5625-8. doi: 10.1073/pnas.85.15.5625.
Hapten- and protein-antigen-specific T helper cells are usually expanded in vitro from lymphocytes obtained from sensitized animals. In this paper we report on the primary activation and proliferation in vitro of T helper cells from nonsensitized animals by using syngeneic cultured epidermal Langerhans cells as a source of potent antigen-presenting cells. The primary in vitro proliferation was blocked with monoclonal antibodies to Ia molecules, to lymphocyte function-associated antigen 1 (LFA-1), and to L3T4. T helper cell populations sensitized in vitro to haptens and protein antigens showed hapten- and antigen-specific proliferation when restimulated in vitro with spleen cells. Besides its experimental usefulness, in vitro generation of syngeneic specific T helper cells may afford possibilities for adoptive immunotherapy.
半抗原和蛋白质抗原特异性T辅助细胞通常在体外由致敏动物的淋巴细胞扩增得到。在本文中，我们报道了通过使用同基因培养的表皮朗格汉斯细胞作为有效的抗原呈递细胞来源，对未致敏动物的T辅助细胞进行体外初次激活和增殖。体外初次增殖被针对Ia分子、淋巴细胞功能相关抗原1（LFA-1）和L3T4的单克隆抗体所阻断。体外对半抗原和蛋白质抗原致敏的T辅助细胞群体在用脾细胞体外再次刺激时表现出半抗原和抗原特异性增殖。除了其实验用途外，同基因特异性T辅助细胞的体外产生可能为过继性免疫治疗提供可能性。
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