白细胞介素-1受体的cDNA表达克隆，免疫球蛋白超家族成员之一。

cDNA expression cloning of the IL-1 receptor, a member of the immunoglobulin superfamily.

作者信息

Sims J E, March C J, Cosman D, Widmer M B, MacDonald H R, McMahan C J, Grubin C E, Wignall J M, Jackson J L, Call S M

机构信息

Immunex Corporation, Seattle, WA 98101.

出版信息

Science. 1988 Jul 29;241(4865):585-9. doi: 10.1126/science.2969618.

DOI:10.1126/science.2969618

PMID:2969618

Abstract

Interleukin-1 alpha and -1 beta (IL-1 alpha and IL-1 beta) are cytokines that participate in the regulation of immune responses, inflammatory reactions, and hematopoiesis. A direct expression strategy was used to clone the receptor for IL-1 from mouse T cells. The product of the cloned complementary DNA binds both IL-1 alpha and IL-1 beta in a manner indistinguishable from that of the native T cell IL-1 receptor. The extracellular, IL-1 binding portion of the receptor is 319 amino acids in length and is composed of three immunoglobulin-like domains. The cytoplasmic portion of the receptor is 217 amino acids long.

摘要

白细胞介素-1α和-1β（IL-1α和IL-1β）是参与免疫反应、炎症反应和造血调节的细胞因子。采用直接表达策略从小鼠T细胞中克隆IL-1受体。克隆的互补DNA产物以与天然T细胞IL-1受体无法区分的方式结合IL-1α和IL-1β。受体的细胞外IL-1结合部分长度为319个氨基酸，由三个免疫球蛋白样结构域组成。受体的细胞质部分长217个氨基酸。