Alcalá-Briseño Ricardo Iván, Okada Ryo, Herrera Favio, Valverde Rodrigo A
Department of Plant Pathology, University of Florida, Gainesville, FL, USA.
Laboratory of Molecular and Cellular Biology, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, 183-8509, Japan.
Arch Virol. 2018 Aug;163(8):2279-2282. doi: 10.1007/s00705-018-3831-9. Epub 2018 Apr 25.
Cluster bean (Cyamopsis tetragonoloba), also called guar, is a drought-tolerant annual legume. We conducted investigations to characterize a large dsRNA (~13-14 kbp) detected in a symptomless cluster bean genotype. The dsRNA was gel-purified and used for Illumina MiSeq sequencing. Reads were assembled, and BLASTx search results showed sequence similarity with viruses classified within the family Endornaviridae. The complete sequence of the putative endornavirus consisted of 12,895 nt and contained an open reading frame which coded for a polyprotein of 4,207 aa with conserved domains for methyltransferase, helicase, and RNA-dependent RNA polymerase. The virus was named cluster bean endornavirus 1 (CBEV-1). A BLASTx search using the polyprotein sequence showed that the closest endornavirus to CBEV-1 was Hordeum vulgare endornavirus.
瓜尔豆（Cyamopsis tetragonoloba），也称为瓜尔，是一种耐旱的一年生豆科植物。我们进行了调查，以表征在一种无症状瓜尔豆基因型中检测到的一种大型双链RNA（约13 - 14 kbp）。该双链RNA经凝胶纯化后用于Illumina MiSeq测序。对读取的序列进行组装，BLASTx搜索结果显示其与归类于内病毒科的病毒具有序列相似性。推定的内病毒的完整序列由12,895个核苷酸组成，包含一个开放阅读框，该开放阅读框编码一个由4,207个氨基酸组成的多聚蛋白，具有甲基转移酶、解旋酶和RNA依赖性RNA聚合酶的保守结构域。该病毒被命名为瓜尔豆内病毒1（CBEV - 1）。使用多聚蛋白序列进行的BLASTx搜索表明，与CBEV - 1最接近的内病毒是大麦内病毒。
