Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, USA.
Department of Plant Pathology and Crop Physiology, Louisiana State University, Baton Rouge, USA.
Arch Virol. 2024 Sep 21;169(10):206. doi: 10.1007/s00705-024-06135-y.
In this study, we completely sequenced the genome of a new member of the genus Alphaendornavirus, family Endornaviridae, from lima bean (Phaseolus lunatus), for which we propose the name "lima bean endornavirus 1" (LbEV1). The complete genome of LbEV1 consists of 15,265 nucleotides, including a stretch of 12 cytosine residues at its 3' end, and contains a long single open reading frame (ORF) coding for a 4980-aa-long polyprotein. Analysis of the polyprotein sequence revealed the presence of four conserved functional domains (in order from the N- to C-terminus): viral helicase 1, peptidase _C97, glycosyltransferase_GTB-type, and viral RNA-dependent RNA polymerase (RdRP). The LbEV1 polyprotein showed the highest amino acid sequence similarity (63% identity and 98% coverage) to Phaseolus vulgaris endornavirus 3 (PvEV3) and also showed 42% identity (95% coverage) to Geranium carolinianum endornavirus. Phylogenetic analysis based on the viral RdRp domain showed that LbEV1 belongs to a subclade within the genus Alphaendornavirus that includes three other viruses infecting plants of the genus Phaseolus.
在这项研究中，我们对来自利马豆（Phaseolus lunatus）的一种新的α内反转录病毒属成员的基因组进行了完全测序，我们将其命名为“利马豆内反转录病毒 1”（LbEV1）。LbEV1 的完整基因组由 15265 个核苷酸组成，其 3' 端有一段 12 个胞嘧啶残基，包含一个长的单一开放阅读框（ORF），编码一个 4980 个氨基酸的多蛋白。对多蛋白序列的分析表明，存在四个保守的功能域（从 N 端到 C 端）：病毒解旋酶 1、肽酶 _C97、糖基转移酶 GTB 型和病毒 RNA 依赖性 RNA 聚合酶（RdRP）。LbEV1 多蛋白与菜豆内反转录病毒 3（PvEV3）表现出最高的氨基酸序列相似性（63%同一性和 98%覆盖度），与天竺葵内反转录病毒的相似性也达到 42%（95%覆盖度）。基于病毒 RdRP 结构域的系统进化分析表明，LbEV1 属于α内反转录病毒属的一个亚群，该亚群还包括另外三种感染菜豆属植物的病毒。
