Department of Biomedical Sciences, School of Biomedical Engineering, Colorado State University, Fort Collins, Colorado 80523, USA.
Lab Chip. 2018 May 15;18(10):1399-1410. doi: 10.1039/c8lc00241j.
This Frontiers review analyzes the rapidly growing microfluidic strategies that have been employed in attempts to create physio relevant 'organ-on-chip' models using primary tissue removed from a body (human or animal). Tissue harvested immediately from an organism, and cultured under artificial conditions is referred to as ex vivo tissue. The use of primary (organotypic) tissue offers unique benefits over traditional cell culture experiments, and microfluidic technology can be used to further exploit these advantages. Defining the utility of particular models, determining necessary constituents for acceptable modeling of in vivo physiology, and describing the role of microfluidic systems in tissue modeling processes is paramount to the future of organotypic models ex vivo. Virtually all tissues within the body are characterized by a large diversity of cellular composition, morphology, and blood supply (e.g., nutrient needs including oxygen). Microfluidic technology can provide a means to help maintain tissue in more physiologically relevant environments, for tissue relevant time-frames (e.g., matching the natural rates of cell turnover), and at in vivo oxygen tensions that can be controlled within modern microfluidic culture systems. Models for ex vivo tissues continue to emerge and grow in efficacy as mimics of in vivo physiology. This review addresses developments in microfluidic devices for the study of tissues ex vivo that can serve as an important bridge to translational value.
这篇 Frontiers 综述分析了快速发展的微流控策略，这些策略旨在使用从人体（人类或动物）中取出的原代组织来创建生理相关的“器官芯片”模型。从生物体中立即收获并在人工条件下培养的组织被称为离体组织。使用原代（器官型）组织具有比传统细胞培养实验更独特的优势，而微流控技术可以进一步利用这些优势。定义特定模型的用途、确定可接受的体内生理学建模所需的成分，以及描述微流控系统在组织建模过程中的作用，对于离体器官型模型的未来至关重要。体内几乎所有的组织都具有细胞组成、形态和血液供应（例如，包括氧气在内的营养需求）的多样性。微流控技术可以提供一种手段，帮助组织在更接近生理的环境中、在更符合生理的时间框架内（例如，匹配细胞更替的自然速率）以及在可通过现代微流控培养系统控制的体内氧张力下生存。离体组织的模型不断涌现并提高其作为体内生理学模拟物的功效。这篇综述介绍了用于离体组织研究的微流控设备的最新进展，这些设备可以作为向转化价值的重要桥梁。
