间充质干细胞中线粒体尺寸的自动化分析：当前方法与未来展望

Automated analysis of mitochondrial dimensions in mesenchymal stem cells: Current methods and future perspectives.

作者信息

Summer Sabrina, Kocsis Agnes, Reihs Eva Ingeborg, Rothbauer Mario, Lonhus Kirill, Stys Dalibor, Ertl Peter, Fischer Michael B

机构信息

Department for Biomedical Research, Center of Experimental Medicine, Danube University Krems, Dr.-Karl-Dorrek-Straße 30, Krems an der Donau, Austria.

Karl Chiari Lab for Orthopaedic Biology & Ludwig Boltzmann Institute for Arthritis and Rehabilitation, Department of Orthopedics and Trauma Surgery, Medical University of Vienna, Währinger Gürtel 18-20, Vienna, Austria.

出版信息

Heliyon. 2023 Jan 18;9(1):e12987. doi: 10.1016/j.heliyon.2023.e12987. eCollection 2023 Jan.

DOI:10.1016/j.heliyon.2023.e12987

PMID:36711314

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9873686/

Abstract

As centre of energy production and key regulators of metabolic and cellular signaling pathways, the integrity of mitochondria is essential for mesenchymal stem cell function in tissue regeneration. Alterations in the size, shape and structural organization of mitochondria are correlated with the physiological state of the cell and its environment and could be used as diagnostic biomarkers. Therefore, high-throughput experimental and computational techniques are crucial to ensure adequate correlations between mitochondrial function and disease phenotypes. The emerge of microfluidic technologies can address the shortcomings of traditional methods to determine mitochondrial dimensions for diagnostic and therapeutic use. This review discusses optical detection methods compatible with microfluidics to measure mitochondrial dynamics and their potential for clinical stem cell research targeting mitochondrial dysfunction.

摘要

作为能量产生的中心以及代谢和细胞信号通路的关键调节因子，线粒体的完整性对于间充质干细胞在组织再生中的功能至关重要。线粒体大小、形状和结构组织的改变与细胞及其环境的生理状态相关，可作为诊断生物标志物。因此，高通量实验和计算技术对于确保线粒体功能与疾病表型之间有足够的相关性至关重要。微流控技术的出现可以弥补传统方法在确定线粒体尺寸用于诊断和治疗方面的不足。本综述讨论了与微流控兼容的光学检测方法，以测量线粒体动力学及其在针对线粒体功能障碍的临床干细胞研究中的潜力。