Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, 02155.
Department of Plastic Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, 15213.
J Biomed Mater Res B Appl Biomater. 2019 Apr;107(3):501-510. doi: 10.1002/jbm.b.34140. Epub 2018 Apr 26.
Biodegradable silk catheters for the delivery of therapeutics are designed with a focus on creating porous gradients that can direct the release of molecules away from the implantation site. Though suitable for a range of applications, these catheters are designed for drug delivery to transplanted adipose tissue in patients having undergone a fat grafting procedure. A common complication for fat grafts is the rapid reabsorption of large volume adipose transplants. In order to prolong volume retention, biodegradable catheters can be embedded into transplanted tissue to deliver nutrients, growth factors or therapeutics to improve adipocyte viability, proliferation, and ultimately extend volume retention. Two fabrication methods are developed: a silk gel-spinning technique, which uses a novel flash-freezing step to induce high porosity throughout the bulk of the tube, and a dip-coating process using silk protein solutions doped with a water soluble porogen. Increased porosity aids in the diffusion of drug through the silk tube in a controllable way. Additionally, we interface the porous tubes with ALZET osmotic pumps for implantation into a subcutaneous nude mouse model. The work described herein will discuss the processing parameters as well as the interfacing between pump and cargo therapeutic and the resulting release profiles. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2018. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 501-510, 2019.
可生物降解的丝质导管用于治疗药物的输送，其设计重点是创建多孔梯度，从而可以将分子的释放从植入部位引导开。尽管这些导管适用于多种应用，但它们是为向接受脂肪移植术的患者的移植脂肪组织中输送药物而设计的。脂肪移植的一个常见并发症是大量脂肪移植的快速再吸收。为了延长体积保留时间，可生物降解的导管可以嵌入移植组织中，以输送营养物质、生长因子或治疗剂，从而提高脂肪细胞的活力、增殖，并最终延长体积保留时间。开发了两种制造方法：一种是丝凝胶纺丝技术，该技术使用新颖的快速冷冻步骤在整个管体中产生高孔隙率；另一种是使用掺杂有水溶性致孔剂的丝蛋白溶液进行浸涂。增加的孔隙率有助于药物通过丝管以可控的方式扩散。此外，我们将多孔管与 ALZET 渗透泵接口，以便植入皮下裸鼠模型中。本文将讨论处理参数以及泵和货物治疗之间的接口，以及由此产生的释放曲线。 © 2018 威利父子公司。生物医学材料研究杂志 B 辑：应用生物材料，2018 年。 © 2018 威利父子公司。生物医学材料研究杂志 B 辑：应用生物材料 107B:501-510，2019 年。
