Rnjak-Kovacina Jelena, Wray Lindsay S, Burke Kelly A, Torregrosa Tess, Golinski Julianne M, Huang Wenwen, Kaplan David L
Department of Biomedical Engineering, Tufts University , 4 Colby Street, Medford, Massachusetts 02155, United States ; Graduate School of Biomedical Engineering, UNSW Australia , Sydney, NSW 2052, Australia.
Department of Biomedical Engineering, Tufts University , 4 Colby Street, Medford, Massachusetts 02155, United States.
ACS Biomater Sci Eng. 2015 Apr 13;1(4):260-270. doi: 10.1021/ab500149p. Epub 2015 Feb 25.
We present a silk biomaterial platform with highly tunable mechanical and degradation properties for engineering and regeneration of soft tissues such as, skin, adipose, and neural tissue, with elasticity properties in the kilopascal range. Lyophilized silk sponges were prepared under different process conditions and the effect of silk molecular weight, concentration and crystallinity on 3D scaffold formation, structural integrity, morphology, mechanical and degradation properties, and cell interactions in vitro and in vivo were studied. Tuning the molecular weight distribution (via degumming time) of silk allowed the formation of stable, highly porous, 3D scaffolds that held form with silk concentrations as low as 0.5% wt/v. Mechanical properties were a function of silk concentration and scaffold degradation was driven by beta-sheet content. Lyophilized silk sponges supported the adhesion of mesenchymal stem cells throughout 3D scaffolds, cell proliferation in vitro, and cell infiltration and scaffold remodeling when implanted subcutaneously in vivo.
我们展示了一种具有高度可调机械性能和降解特性的丝绸生物材料平台，用于工程化和再生软组织，如皮肤、脂肪和神经组织，其弹性性能在千帕范围内。在不同工艺条件下制备了冻干丝绸海绵，并研究了丝绸分子量、浓度和结晶度对三维支架形成、结构完整性、形态、机械和降解性能以及体内外细胞相互作用的影响。通过调节丝绸的分子量分布（通过脱胶时间），可以形成稳定、高度多孔的三维支架，即使丝绸浓度低至0.5% wt/v时也能保持形状。机械性能是丝绸浓度的函数，支架降解由β-折叠含量驱动。冻干丝绸海绵在整个三维支架中支持间充质干细胞的黏附、体外细胞增殖，以及在体内皮下植入时的细胞浸润和支架重塑。
