State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, School of Life Sciences , Nanjing University , Nanjing 210023 , China.
Department of Clinical Laboratory , The First Affiliated Hospital of Nanjing Medical University , Nanjing 210029 , China.
Org Lett. 2018 May 18;20(10):2967-2971. doi: 10.1021/acs.orglett.8b01006. Epub 2018 Apr 26.
Streptoseomycin (1), which is a rare macrodilactone with potent activities against microaerophilic bacteria, featuring a pentacyclic 5/14/10/6/6 ring system together with an ether bridge, was characterized by a combination of spectroscopic method and X-ray analysis from a marine Streptomyces seoulensis. Sequencing and characterization of a ∼76-kb biosynthetic gene cluster led to the proposition of the biosynthetic pathway of 1. Heterologous expression of the gene cluster using a BAC vector in Streptomyces chartreusis 1018 led to the successful production of 1.
链丝菌素（Streptoseomycin，1）是一种罕见的大环内酯类化合物，对微需氧菌具有很强的活性，其特征是具有五圆环系统（5/14/10/6/6）和一个醚桥。它是从海洋链霉菌（Streptomyces seoulensis）中通过光谱方法和 X 射线分析相结合而鉴定的。通过对约 76kb 的生物合成基因簇进行测序和表征，提出了 1 的生物合成途径。使用 BAC 载体在绛红链霉菌（Streptomyces chartreusis 1018）中异源表达该基因簇，成功地生产出了 1。
