1 Department of Botany and Plant Pathology and Genome Research and Biocomputing, Oregon State University, Corvallis, OR 97331-2902, U.S.A.
2 Columbia Basin Agricultural Research Center, Oregon State University, Adams, OR 97810, U.S.A.; and.
Mol Plant Microbe Interact. 2018 Oct;31(10):1069-1074. doi: 10.1094/MPMI-12-17-0328-R. Epub 2018 Sep 6.
Cochliobolus victoria, the causal agent of Victoria blight, is pathogenic due to its production of a toxin called victorin. Victorin sensitivity in oats, barley, Brachypodium spp., and Arabidopsis has been associated with nucleotide-binding site leucine-rich repeat (NLR) genes, a class of genes known for conferring disease resistance. In this work, we investigated the sensitivity of Phaseolus vulgaris to victorin. We found that victorin sensivity in Phaseolus vulgaris is a developmentally regulated, quantitative trait. A single quantitative trait locus (QTL) accounted for 34% of the phenotypic variability in victorin sensitivity among Stampede × Red Hawk (S×R) recombinant inbred lines. We cloned two NLR-encoding genes within this QTL and showed one, Phvul05G031200 (PvLOV), confers victorin-dependent cell death when overexpressed in Nicotiana benthamiana. Protein sequences of PvLOV from victorin-sensitive and the victorin-resistant bean parents differ by two amino acids in the leucine-rich repeat region, but both proteins confer victorin-dependent cell death when overexpressed in N. benthamiana.
角斑病菌（Cochliobolus victoria）是维多利亚叶枯病的病原体，其致病原因是产生一种名为维多利亚毒素的毒素。燕麦、大麦、短柄草属（Brachypodium spp.）和拟南芥对维多利亚毒素的敏感性与核苷酸结合位点富含亮氨酸重复（NLR）基因有关，NLR 基因是一类赋予抗病性的基因。在这项工作中，我们研究了菜豆对维多利亚毒素的敏感性。我们发现菜豆对维多利亚毒素的敏感性是一个发育调控的数量性状。在 Stampede × Red Hawk（S×R）重组自交系中，一个单一位点 QTL 解释了维多利亚毒素敏感性表型变异的 34%。我们在这个 QTL 内克隆了两个编码 NLR 的基因，并表明其中一个，Phvul05G031200（PvLOV），在本氏烟中过表达时会导致维多利亚毒素依赖性细胞死亡。来自对维多利亚毒素敏感和抗维多利亚毒素的菜豆亲本的 PvLOV 蛋白序列在富含亮氨酸重复区有两个氨基酸的差异，但这两种蛋白在本氏烟中过表达时都会导致维多利亚毒素依赖性细胞死亡。
