Tsertsvadze A, Ebralidze L, Berashvili D, Matchutadze I, Bakuridze A
Tbilisi State Medical University, Department of pharmaceutical technology; GM Pharmaceuticals LTD, Georgia.
Georgian Med News. 2018 Mar(276):182-186.
The objective of the research was development extraction process of humic substances from sphagnum peat peloids, selection of extragent and characterization of humic substances. The objects of the research: Kolkheti peat peloids (Ispani, Anaklia, Churia, Chirukhi, Peranga) of different formation phases. Research was held using modern instrumental methods of analysis: UV spectrophotometer, Scanning Electron Microscopy, Centrifuge, Dry oven, Ultraturax. In the research extraction process of humic substances from sphagnum peat peloids was developed and composition of humic substances was studied, also E4/E6 humification coefficient was evaluated. Based on the results extraction conditions of humic substances from the peat peloids samples were determined: a) extragent with maximum yield - 1.0N NaOH; b) mixing type - KA-ULTRA TURAX-T18 - 20 000 rpm/min; c) Precipitant of humic substances -10% HCL. Composition of humic substances are studied in the samples and their relatively high content is determined in Anaklia and Churia sphagnum peat peloids. For characterization of humic substances E4/E6 humification coefficient was evaluated. Low ration of E4/E6 < 5 was established in anaklia, churia and ispani peat peloids. High ration of E4/E6 < 10 coefficient is determined in chirukhi and peranga peat peloids.
该研究的目的是开发从泥炭藓泥炭球粒中提取腐殖物质的工艺、选择萃取剂并对腐殖物质进行表征。研究对象为不同形成阶段的科尔基蒂泥炭球粒(伊斯帕尼、阿纳克利、楚里亚、奇鲁希、佩兰加)。研究采用现代仪器分析方法进行:紫外分光光度计、扫描电子显微镜、离心机、干燥箱、超高速均质机。在研究中,开发了从泥炭藓泥炭球粒中提取腐殖物质的工艺,研究了腐殖物质的组成,还评估了E4/E6腐殖化系数。根据研究结果,确定了从泥炭球粒样品中提取腐殖物质的条件:a)产率最高的萃取剂——1.0N氢氧化钠;b)混合类型——KA-ULTRA TURAX-T18——20000转/分钟;c)腐殖物质沉淀剂——10%盐酸。对样品中腐殖物质的组成进行了研究,确定在阿纳克利和楚里亚泥炭藓泥炭球粒中其含量相对较高。为了表征腐殖物质,评估了E4/E6腐殖化系数。在阿纳克利、楚里亚和伊斯帕尼泥炭球粒中确定了较低的E4/E6比值<5。在奇鲁希和佩兰加泥炭球粒中确定了较高的E4/E6系数比值<10。