Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China.
Angew Chem Int Ed Engl. 2018 Sep 10;57(37):11882-11887. doi: 10.1002/anie.201803324. Epub 2018 May 16.
Protein biomarkers in blood have been widely used in the early diagnosis of disease. However, simultaneous detection of many biomarkers in a single sample remains challenging. Herein, we show that the combination of a sandwich assay and DNA-assisted nanopore sensing could unambiguously identify and quantify several antigens in a mixture. We use five barcode DNAs to label different gold nanoparticles that can selectively bind specific antigens. After the completion of the sandwich assay, barcode DNAs are released and subject to nanopore translocation tests. The distinct current signatures generated by each barcode DNA allow simultaneous quantification of biomarkers at picomolar level in clinical samples. This approach would be very useful for accurate and multiplexed quantification of cancer-associated biomarkers within a very small sample volume, which is critical for non-invasive early diagnosis of cancer.
血液中的蛋白质生物标志物已被广泛用于疾病的早期诊断。然而，在单个样本中同时检测多种生物标志物仍然具有挑战性。在此，我们展示了夹心测定法和 DNA 辅助纳米孔传感的组合可明确识别和定量混合物中的多种抗原。我们使用 5 种条形码 DNA 标记可选择性结合特定抗原的不同金纳米颗粒。夹心测定完成后，条形码 DNA 被释放并进行纳米孔迁移测试。每个条形码 DNA 产生的独特电流特征允许在临床样本中以皮摩尔级别的精度同时定量生物标志物。这种方法对于在非常小的样本量中准确且多重定量与癌症相关的生物标志物非常有用，这对于癌症的非侵入性早期诊断至关重要。
