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[单次给予工业高温设施灰烬后大鼠肺中酸性磷酸酶(AcP[E.C. 3.1.3.2.])和三磷酸腺苷酶(ATPase[E.C. 3.6.1.3.])活性的测定]

[Determining acid phosphatase (AcP[E.C. 3.1.3.2.]) and adenosine triphosphatase (ATPase [E.C. 3.6.1.3.]) activity in the lungs of rats following a single administration of ashes from industrial high-heat facilities].

作者信息

Romanowska-Sarlej J, Staszyc J, Królikowska-Prasał I, Jedrzejewska E, Kifer E, Matysiak W

机构信息

Abteilung für Histologie und Embryologie der Medizinischen, Akademie in Lubin.

出版信息

Gegenbaurs Morphol Jahrb. 1988;134(1):67-77.

PMID:2969838
Abstract

There was examined pulmonary tissue of white rats, which had been administered intratrachealy a single dose of the respirable fraction of ashes sample from 6 different power stations elektrohasting plants and hasting plants in Poland (0.2 ml suspension; 50 mg of the examined sample in 0.6 cm3 of NaCl solution). 9 months after the application of the ashes, biopsies of the left lung were taken and there was determined the activity of acid phosphatase (AcP) and adenosinetriphosphatase (ATP-ase) histoenzymatically. There was found sensitivity of these hydrolases and changes of their activity connected with chemical composition of the examined ashes.

摘要

对经气管内给予来自波兰6个不同发电站、电炉厂和冶炼厂的灰分样品可吸入部分单剂量的白色大鼠肺组织进行了检查(0.2毫升悬浮液;0.6立方厘米氯化钠溶液中含50毫克受试样品)。在施加灰分9个月后,取左肺活检组织,并通过组织酶学方法测定酸性磷酸酶(AcP)和三磷酸腺苷酶(ATP酶)的活性。发现这些水解酶具有敏感性,且其活性变化与受试灰分的化学成分有关。

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