Faculty of Veterinary Medicine, Università degli Studi di Teramo, Italy.
Azienda USL di Reggio Emilia, Reggio Emilia, Italy.
Virus Res. 2018 Jun 2;251:1-5. doi: 10.1016/j.virusres.2018.04.014. Epub 2018 Apr 24.
Caliciviruses of the Sapovirus genus have been recently detected in dogs. Canine sapoviruses (SaVs) have been identified in the stools of young or juvenile animals with gastro-enteric disease at low prevalence (2.0-2.2%), but whether they may have a role as enteric pathogens and to which extent dogs are exposed to SaVs remains unclear. Here, we report the expression in a baculovirus system of virus like-particles (VLPs) of a canine SaV strain, the prototype virus Bari/4076/2007/ITA. The recombinant antigen was used to develop an enzyme-linked immunosorbent assay (ELISA). By screening an age-stratified collection of serum samples from 516 dogs in Italy, IgG antibodies specific for the canine SaV VLPs were detected in 40.3% (208/516) of the sera. Also, as observed for SaV infection in humans, we observed a positive association between seropositivity and age, with the highest prevalence rates in dogs older than 4 years of age.
最近在狗中检测到杯状病毒属的诺如病毒。已在患有胃肠疾病的幼小动物的粪便中鉴定出犬诺如病毒（SaV），其流行率较低（2.0-2.2%），但它们是否可能作为肠道病原体以及狗暴露于 SaV 的程度仍不清楚。在这里，我们报告了在杆状病毒系统中表达一种犬诺如病毒株（原型病毒 Bari/4076/2007/ITA）的病毒样颗粒（VLPs）。该重组抗原被用于开发酶联免疫吸附测定（ELISA）。通过对来自意大利的 516 只狗的按年龄分层的血清样本进行筛选，在 516 份血清中的 40.3%（208/516）中检测到针对犬诺如病毒 VLPs 的 IgG 抗体。此外，与人类的 SaV 感染一样，我们观察到血清阳性与年龄之间存在正相关，4 岁以上的狗的阳性率最高。
