Di Martino Barbara, Di Profio Federica, Melegari Irene, Sarchese Vittorio, Massirio Ivano, Palermo Giuseppe, Romito Giovanni, Lorusso Eleonora, Lanave Gianvito, Bodnar Livia, Buonavoglia Canio, Marsilio Fulvio, Green Kim Y, Martella Vito
Faculty of Veterinary Medicine, Università degli Studi di Teramo, Italy.
Faculty of Veterinary Medicine, Università degli Studi di Teramo, Italy.
Vet Microbiol. 2017 May;203:68-72. doi: 10.1016/j.vetmic.2017.03.006. Epub 2017 Mar 4.
Molecular and serological data suggest that noroviruses (NoVs) might be transmitted between humans and domestic carnivores. In this study we screened an age-stratified collection of canine sera (n=516) by using an ELISA assay based on virus-like particles (VLPs) of human NoVs GII.4 and GIV.1 and carnivore NoVs GIV.2 and GVI.2. Antibodies against GII.4 and GIV.1 human NoVs and GIV.2 and GVI.2 NoVs from carnivores were identified in dog sera (13.0%, 67/516) suggesting their exposure to homologous and heterologous NoVs. Analysis of the trends of age-class prevalence showed a gradual increase in the positive rate from 9.0% and 7.0%, in young dogs <1year of age to 15.0% in dogs older than 12 years, for GII.4 and GVI.2 NoVs, respectively. A significant difference in the IgG distribution by age classes was observed for GIV.1 NoVs, with the highest rate of antibodies (7.0%) in the age group <1year and the lowest (1.0%) in the age-classes 7-9 (P=0.049). High correlation between the reactivity to GII.4 and GVI.2 NoVs was observed, likely due to conserved epitopes in the capsid structure.
分子和血清学数据表明，诺如病毒（NoVs）可能在人类和家养食肉动物之间传播。在本研究中，我们使用基于人诺如病毒GII.4和GIV.1以及食肉动物诺如病毒GIV.2和GVI.2的病毒样颗粒（VLPs）的ELISA检测法，对按年龄分层收集的犬血清（n = 516）进行了筛查。在犬血清中（13.0%，67/516）鉴定出了针对人诺如病毒GII.4和GIV.1以及来自食肉动物的诺如病毒GIV.2和GVI.2的抗体，这表明它们接触过同源和异源诺如病毒。年龄组患病率趋势分析显示，对于GII.4和GVI.2诺如病毒，阳性率分别从1岁以下幼犬的9.0%和7.0%逐渐增加到12岁以上犬的15.0%。对于GIV.1诺如病毒，观察到不同年龄组的IgG分布存在显著差异，1岁以下年龄组的抗体率最高（7.0%），7 - 9岁年龄组最低（1.0%）（P = 0.049）。观察到对GII.4和GVI.2诺如病毒的反应性之间存在高度相关性，这可能是由于衣壳结构中存在保守表位。
