Laboratorio de Alimentos, División de Desarrollo Biotecnológico, Departamento de Ciencias Médicas y de la Vida, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Linda Vista, 47820 Ocotlán, Jalisco 47820, Mexico.
Laboratorio de Microbiología, División de Desarrollo Biotecnológico, Departamento de Ciencias Médicas y de la Vida, Centro Universitario de la Ciénega, Universidad de Guadalajara, Av. Universidad 1115, Col. Linda Vista, 47820 Ocotlán, Jalisco 47820, Mexico.
J Glob Antimicrob Resist. 2018 Sep;14:197-201. doi: 10.1016/j.jgar.2018.04.010. Epub 2018 Apr 23.
The effectiveness of disinfectants can vary according to the microorganism, type of residues and surface. The aim of this study was to determine the effectiveness of four disinfectants in the presence of organic matter and their residual effect on stainless steel grade 304 (SS) and polypropylene B (PP-B).
The effectiveness of the disinfectants in the presence of meat extract, egg yolk and whole milk was determined according to AOAC and UNE-EN 1040:2015, and the residual effect was determined according to UNE-EN 13697:2015 using approved strains.
The effectiveness of the disinfectants was affected to different degrees depending on the organic matter present. SANICIP Q5 [400μg/mL; fifth-generation quaternary ammonium compound (QAC)] was most effective in the presence of 10% meat extract, whilst SANICIP PAA (200μg/mL; peracetic acid) showed better activity in the presence of 10% egg yolk and whole milk. In the evaluation of residual effect on SS and PP-B, the QAC had a better effect, reducing Listeria monocytogenes ATCC 19111 by 6 Log CFU/mL at 24h after its application. Conversely, the disinfectants had no residual effect against Pseudomonas aeruginosa ATCC 15442.
The antimicrobial activity of the disinfectants tested against pathogenic and spoilage microorganisms was affected according to the type of organic matter present. SANICIP Q5 had a greater residual effect than the other disinfectants evaluated. Moreover, the residual effect of a disinfectant is greater on SS than on PP-B and is dependent on the microorganism tested.
消毒剂的有效性可能因微生物、残留物类型和表面而异。本研究旨在确定四种消毒剂在存在有机物的情况下的有效性及其对 304 级不锈钢（SS）和聚丙烯 B（PP-B）的残留效果。
根据 AOAC 和 UNE-EN 1040:2015 测定了肉浸液、蛋黄和全脂牛奶存在下消毒剂的有效性，根据 UNE-EN 13697:2015 使用批准的菌株测定了残留效果。
消毒剂的有效性因存在的有机物而受到不同程度的影响。SANICIP Q5[400μg/mL；第五代季铵盐（QAC）]在存在 10%肉浸液的情况下效果最佳，而 SANICIP PAA（200μg/mL；过氧乙酸）在存在 10%蛋黄和全脂牛奶的情况下表现出更好的活性。在 SS 和 PP-B 上残留效果的评估中，QAC 的效果更好，在应用后 24 小时将单核细胞增生李斯特菌 ATCC 19111 减少了 6 Log CFU/mL。相反，消毒剂对铜绿假单胞菌 ATCC 15442 没有残留效果。
测试的消毒剂对致病菌和腐败微生物的抗菌活性根据存在的有机物类型而有所不同。SANICIP Q5 比评估的其他消毒剂具有更大的残留效果。此外，消毒剂的残留效果在 SS 上大于 PP-B，并且取决于测试的微生物。
