Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India; Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India.
Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India; Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, New Delhi, India.
Infect Genet Evol. 2018 Aug;62:253-261. doi: 10.1016/j.meegid.2018.04.030. Epub 2018 Apr 24.
Acute gastroenteritis due to Rotavirus (RV) infection is a major cause of morbidity and mortality in infants and young children worldwide. In India, around 0.1 million death reported annually due to RV illness. So, to assess the disease burden continuous surveillance of the circulating genotypes is needed. This study aimed to ascertain the genetic variance of 429 rotavirus positive specimens observed during the period October 2013-September 2014 at four study centers from North India. Out of 1057 patients enrolled, 1018 stool samples were collected at four centers in four different states of North India. Children aged <5 years who showed the symptoms of severe diarrhea and needed hospitalization were enrolled. The stool samples collected were screened by Enzyme Immuno Assay (EIA), and the RV positive samples were characterized by semi-nested PCR. During the study period October 2013 through September 2014, ~42% patients were found to be rotavirus positive of 1018 collected specimen. In Delhi, Rohtak and Meerut, we observed that Rotavirus is seasonal compared to Tanda (HP). The rate of rotavirus detection was significantly higher among children aged below 2 years, and a total of 21.5% of rotavirus infections comprised children aged <6 months. Genotype G1(48.0%) was predominant and frequently circulating strain whereas G12 (16.8%) and G9 (10.0%) was second and third prevalent strain in the four states of North India. High frequency of G1 genotypes was detected under the age group of 6-11 months which is followed by G12, similarly high rate severe disease was observed due to G1 genotypes followed by P[8], P[6] and G12. The most common types of strains were G1P[8] (27.73% of strains), G12P[6] (13.28%), G9P[4] (7.23%) and G1P[6] (6.75%). The rare strain reported were G1P[9]; P[11] strain was detected in combination with G1, G2, and G12. These data emphasized G12 is the second most predominant strain circulating among Northern Indian children highlights the needs for inclusion in the future polyvalent vaccine to break the burden of rotavirus infection.
轮状病毒（RV）感染引起的急性肠胃炎是全球婴幼儿发病率和死亡率的主要原因。在印度，每年约有 0.1 万人因 RV 疾病而死亡。因此，为了评估疾病负担，需要对循环基因型进行持续监测。本研究旨在确定 2013 年 10 月至 2014 年 9 月期间在印度北部四个研究中心观察到的 429 份轮状病毒阳性标本的遗传变异。在纳入的 1057 名患者中，在印度北部四个不同邦的四个中心采集了 1018 份粪便样本。纳入患有严重腹泻且需要住院的＜5 岁儿童。收集的粪便样本采用酶免疫测定（EIA）进行筛查，RV 阳性样本采用半巢式 PCR 进行特征分析。在 2013 年 10 月至 2014 年 9 月期间，我们发现 1018 份采集的样本中约有 42%为轮状病毒阳性。在德里、罗塔克和密鲁特，我们观察到轮状病毒具有季节性，而坦达（HP）则没有。在年龄＜2 岁的儿童中，轮状病毒的检出率显著较高，总共有 21.5%的轮状病毒感染发生在＜6 个月的儿童中。基因型 G1（48.0%）是主要和频繁循环的菌株，而 G12（16.8%）和 G9（10.0%）是北印度四个邦的第二和第三常见菌株。在 6-11 个月年龄组中检测到高频 G1 基因型，其次是 G12，同样由于 G1 基因型导致严重疾病的发生率较高，其次是 P[8]、P[6]和 G12。最常见的菌株类型是 G1P[8]（27.73%的菌株）、G12P[6]（13.28%）、G9P[4]（7.23%）和 G1P[6]（6.75%）。报告的罕见菌株有 G1P[9]；还检测到 P[11]株与 G1、G2 和 G12 结合。这些数据强调 G12 是印度北部儿童中循环的第二大主要菌株，这突出表明需要将其纳入未来的多价疫苗中，以减轻轮状病毒感染的负担。
