糖尿病肾病患者肾小球唾液酸的双重免疫荧光研究。
Double immunofluorescence studies of glomerular sialic acids in patients with diabetic nephropathy.
作者信息
Tomino Y, Yagame M, Inoue W, Watanabe S, Kaneshige H, Nomoto Y, Sakai H, Takeda M
机构信息
Department of Internal Medicine, School of Medicine, Tokai University, Isehara City, Japan.
出版信息
J Diabet Complications. 1987 Apr-Jun;1(2):61-4. doi: 10.1016/s0891-6632(87)80082-x.
A study of double immunofluorescence staining of sialic acids and IgG in the glomeruli from patients with diabetic nephropathy is described. Renal biopsy specimens from seven patients with diabetic nephropathy were stained with rhodamine-labeled tricum vulgaris (WGA), limulus polyphemus (LPA), and FITC-labeled anti-human IgG antiserum. Both binding of WGA and deposition of IgG in glomerular capillary walls were observed in all patients with diabetic nephropathy. However, the binding of LPA in tissue from the same patients was minimal, although deposition of IgG was marked. It is suggested that an accumulation of substances with an affinity for WGA (most probably N-acetyl glucosamine or N-acetyl neuraminic acid) occurs in the glomerular capillary walls of patients with diabetic nephropathy.
本文描述了一项对糖尿病肾病患者肾小球中唾液酸和IgG进行双重免疫荧光染色的研究。对7例糖尿病肾病患者的肾活检标本用罗丹明标记的普通小麦凝集素（WGA）、鲎试剂（LPA）以及异硫氰酸荧光素标记的抗人IgG抗血清进行染色。在所有糖尿病肾病患者中均观察到WGA的结合以及IgG在肾小球毛细血管壁的沉积。然而，尽管IgG沉积明显，但同一患者组织中LPA的结合极少。提示在糖尿病肾病患者的肾小球毛细血管壁中存在对WGA具有亲和力的物质（很可能是N-乙酰葡糖胺或N-乙酰神经氨酸）的蓄积。
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