Tomino Y, Inoue W, Watanabe S, Yagame M, Eguchi K, Nomoto Y, Sakai H
Department of Internal Medicine, School of Medicine, Tokai University, Kanagawa-Ken, Japan.
Am J Nephrol. 1988;8(1):21-6. doi: 10.1159/000167548.
A study on immunofluorescence of sialic acids in glomeruli from patients with diabetic nephropathy is described. Measurement of sialic acid in sera from 25 patients with diabetes mellitus was also performed. Renal biopsy specimens from 12 patients with diabetic nephropathy were stained with FITC-labeled antihuman IgG antiserum and rhodamine-labeled Triticum vulgaris (WGA) or Limulus polyphemus (LPA). These specimens were also stained with such reagents after treatment with neuraminidase, trypsin or citrate buffer. Both deposition of IgG and binding of WGA in the glomerular capillary walls were observed in all patients with diabetic nephropathy. The binding of WGA in the glomerular capillary walls in diabetic nephropathy was significantly increased compared with that in four normal renal tissues. However, the binding of LPA was hardly observed in the glomerular capillary walls of patients with diabetic nephropathy. The binding of WGA in the glomeruli was markedly decreased after treatment with neuraminidase although it was hardly decreased after treatment with trypsin or citrate buffer. The levels of sialic acid in sera from patients with diabetic nephropathy were markedly increased. It is suggested that accumulated substances in the glomerular capillary walls with an affinity for WGA are mainly composed of N-acetyl glucosamine and/or N-acetyl neuraminic acid in patients with diabetic nephropathy.
本文描述了一项关于糖尿病肾病患者肾小球中唾液酸免疫荧光的研究。同时对25例糖尿病患者血清中的唾液酸进行了测定。用异硫氰酸荧光素（FITC）标记的抗人IgG抗血清和罗丹明标记的普通小麦（WGA）或鲎试剂（LPA）对12例糖尿病肾病患者的肾活检标本进行染色。这些标本在用神经氨酸酶、胰蛋白酶或柠檬酸盐缓冲液处理后，也用上述试剂进行染色。在所有糖尿病肾病患者中均观察到IgG在肾小球毛细血管壁的沉积以及WGA的结合。与四个正常肾组织相比，糖尿病肾病患者肾小球毛细血管壁中WGA的结合显著增加。然而，在糖尿病肾病患者的肾小球毛细血管壁中几乎未观察到LPA的结合。用神经氨酸酶处理后，肾小球中WGA的结合明显减少，而用胰蛋白酶或柠檬酸盐缓冲液处理后几乎未减少。糖尿病肾病患者血清中的唾液酸水平明显升高。提示糖尿病肾病患者肾小球毛细血管壁中对WGA有亲和力的积聚物质主要由N - 乙酰葡糖胺和/或N - 乙酰神经氨酸组成。
