Wei Quanwei, Shi Fangxiong, He Jianwen, Xie Chong, Xu Ke, Zhang Wei, Sun Siyu, Fedail Jaafar, Watanabe Gen, Taya Kazuyoshi
College of Animal Science and Technology Nanjing Agricultural University Nanjing People's Republic of China.
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture Tokyo University of Agriculture and Technology Tokyo Japan.
Reprod Med Biol. 2012 Mar 7;11(3):135-141. doi: 10.1007/s12522-012-0122-0. eCollection 2012 Jul.
The study was aimed to examine the effects of exogenous 17β-estradiol (E) on oocyte nest breakdown and follicular growth in the ovary of neonatal and immature mice.
The effect of E on primordial follicle formation and differentiation were examined by treating neonatal mice with E in mineral oil at a dose of 10 mg/kg by sc injection each day from postnatal days 1 (PND1) to 10 (PND10). This investigation was conducted by using histological observation and immunohistochemical staining of proliferating cell nuclear antigen (PCNA); the numbers of follicles at each stage were examined to assess the follicular development.
The results indicated that such dose of E inhibited oocyte nest breakdown from PND1 to PND10 significantly and enhanced the oocyte staining intensity of PCNA in the ovary by PND3, 5 and 10. PCNA staining was mainly detected on growing follicles (stages later than primary follicle). Our results also revealed that E decreased the size of the reproductive tract at PD10.
This dose of E treatment inhibits oocyte nest breakdown and primordial follicle assembly, and causes follicle losses after PND5. Effects of exogenous E on follicular development in the neonatal and immature mouse may through a PCNA-dependent pathway.
本研究旨在探讨外源性17β-雌二醇（E）对新生和未成熟小鼠卵巢中卵母细胞巢破裂及卵泡生长的影响。
通过从出生后第1天（PND1）至第10天（PND10）每天以10 mg/kg的剂量经皮下注射将E溶解于矿物油中处理新生小鼠，来检测E对原始卵泡形成和分化的影响。本研究通过组织学观察和增殖细胞核抗原（PCNA）免疫组化染色进行；检查各阶段卵泡数量以评估卵泡发育情况。
结果表明，该剂量的E从PND1至PND10显著抑制卵母细胞巢破裂，并在PND3、5和10时增强卵巢中PCNA的卵母细胞染色强度。PCNA染色主要在生长卵泡（初级卵泡之后的阶段）中检测到。我们的结果还显示，E在PD10时减小了生殖道的大小。
该剂量的E处理抑制卵母细胞巢破裂和原始卵泡组装，并在PND5后导致卵泡丢失。外源性E对新生和未成熟小鼠卵泡发育的影响可能通过PCNA依赖途径。
