Tsujii H, Lee J H, Hossain M S, Tareq K M A, Hamano K, Sawada T
Laboratory of Animal Biotechnology Shinshu University Minamiminowa-mura 399-4598 Nagano Japan.
Nagoya Reproduction Center The Sawada Women's Clinic Chikusaku Nagoya Aichi Japan.
Reprod Med Biol. 2008 Dec 13;8(1):19-24. doi: 10.1007/s12522-008-0003-8. eCollection 2009 Mar.
This study was conducted to investigate the effects of supplementing fructose in the culture medium on in vitro maturation (IVM), in vitro fertilization (IVF), and metabolism of porcine oocytes.
Porcine oocytes were matured in vitro in modified North Carolina State University 37 medium (NCSU-37) and then supplemented with either glucose (5.5 mM), fructose (5.5 mM), or glucose (2.75 mM) plus fructose (2.75 mM). The maturation and fertilization of oocytes, and the incorporation and oxidation of C-glucose, C-fructose, and C-methionine in oocytes at different stages of development were examined.
The supplementation of glucose plus fructose significantly promoted ( < 0.05) oocytes germinal vesicle break down (GVBD), maturation to metaphase II (MII), penetration by spermatozoa, and male pronuclear formation compared with glucose. The incorporation and oxidation of C-methionine into the oocyte significantly increased ( < 0.05) with glucose plus fructose supplementation than glucose. A significantly higher ( < 0.05) rate of incorporation and oxidation was achieved with C-fructose compared to C-glucose.
Glucose plus fructose supplementation improved maturation, penetration by spermatozoa, male pronuclear formation, and energy metabolism by porcine oocytes.
本研究旨在探讨在培养基中添加果糖对猪卵母细胞体外成熟（IVM）、体外受精（IVF）及代谢的影响。
猪卵母细胞在改良的北卡罗来纳州立大学37号培养基（NCSU - 37）中进行体外成熟培养，然后分别添加葡萄糖（5.5 mM）、果糖（5.5 mM）或葡萄糖（2.75 mM）加果糖（2.75 mM）。检测卵母细胞的成熟和受精情况，以及不同发育阶段卵母细胞中C - 葡萄糖、C - 果糖和C - 蛋氨酸的掺入及氧化情况。
与单独添加葡萄糖相比，添加葡萄糖加果糖显著促进（<0.05）了卵母细胞的生发泡破裂（GVBD）、成熟至中期II（MII）、精子穿透及雄原核形成。添加葡萄糖加果糖时，C - 蛋氨酸掺入卵母细胞的量及氧化量比单独添加葡萄糖时显著增加（<0.05）。与C - 葡萄糖相比，C - 果糖的掺入及氧化率显著更高（<0.05）。
添加葡萄糖加果糖可改善猪卵母细胞的成熟、精子穿透、雄原核形成及能量代谢。
