Tsujii Hirotada, Ohta Emi, Miah Abdul Gaffar, Hossain Sharoare, Salma Ummay
Laboratory of Animal Biotechnology, Shinshu University, Minamiminowa-mura Nagano, Japan.
Reprod Med Biol. 2006 Nov 23;5(4):255-261. doi: 10.1111/j.1447-0578.2006.00150.x. eCollection 2006 Dec.
The present study was carried out to investigate the effects of fructose supplementation in glucose containing mTALP medium on motility, acrosome reaction and fertilization capability of boar spermatozoa. Boar spermatozoa were preincubated, swum-up, resuspended and then incubated for 6 h in mTALP medium supplemented with 0, 0.5, or 1.0 mmol fructose in the presence of 5.0 mmol glucose. After completion of the specified incubation period, motility was determined subjectively on the basis of speed of progression and on the type of forward movement of spermatozoa; acrosome status was evaluated by applying a triple staining technique; and fertilization capability was assessed by acetic-orcein staining. The combination of fructose and glucose (0.5 + 5.0 mmol) supplements in mTALP medium improved sperm motility significantly ( 0.05), more than glucose alone (5.0 mmol) at 2-6 h of incubation. Acrosome reaction (live spermatozoa) and the sperm penetration rate was increased significantly ( 0.05) when the spermatozoa were treated with the combination of fructose and glucose compared with glucose alone, but the incidence of polyspermy was not significantly different between the treatments. These results suggest that the combination of glucose and fructose as supplements in mTALP medium improve the progressive motility, acrosome reaction and fertilization capability of boar spermatozoa. (Reprod Med Biol 2006; 255-261).
本研究旨在探讨在含葡萄糖的mTALP培养基中添加果糖对猪精子活力、顶体反应及受精能力的影响。猪精子经预孵育、上浮、重悬后，在含有5.0 mmol葡萄糖的mTALP培养基中分别添加0、0.5或1.0 mmol果糖，孵育6小时。在规定的孵育期结束后，根据精子前进速度和前进运动类型主观测定精子活力；采用三重染色技术评估顶体状态；通过醋酸洋红染色评估受精能力。在mTALP培养基中添加果糖和葡萄糖（0.5 + 5.0 mmol）的组合在孵育2至6小时时显著提高了精子活力（P < 0.05），比单独添加葡萄糖（5.0 mmol）效果更好。与单独使用葡萄糖相比，用果糖和葡萄糖组合处理精子时，顶体反应（活精子）和精子穿透率显著提高（P < 0.05），但多精受精发生率在各处理之间无显著差异。这些结果表明，在mTALP培养基中添加葡萄糖和果糖的组合可提高猪精子的渐进性活力、顶体反应及受精能力。（《生殖医学与生物学》2006年；255 - 261页）
