常规囊胚培养与移植：201例患者的经验
Routine blastocyst culture and transfer: 201 patients' experience.
作者信息
Hsieh Y Y, Tsai H D, Chang F C
机构信息
Department of Obstetrics and Gynecology, China Medical College Hospital, Taichung, Taiwan.
出版信息
J Assist Reprod Genet. 2000 Sep;17(8):405-8. doi: 10.1023/a:1009458215962.
PURPOSE
The purpose was to compare the outcomes between day-5 blastocyst and day-2 embryo transfers.
METHODS
Infertile women who accepted the ovarian hyperstimulation and oocyte retrieval were divided: Group 1, day 2 embryo transfer, group 2, cultured to day 5 in serum-free sequential culture medium and transfer. Early embryo quality and growth, blastocyst formation and quality, implantation rate (IR) and pregnancy rates (PR) were detected.
RESULTS
Total blastocyst formation rate was 49.4%. Better early embryo quality (days 2, 3) and higher day 3 blastomere number possess higher blastocyst formation rate. The IR for day 2 and day 5 embryos were 10.8% and 22.2%, respectively. The PR in both groups were comparable (37.3% vs. 41.8%).
CONCLUSIONS
Blastocyst transfer has higher IR and comparable PR as those of day 2 embryo transfer. Early embryo qualities and day 3 blastomere number are useful in predicting the final blastocyst formation. Blastocyst formation rate is not related to maternal age, infertile causes, insemination methods, and early embryo number.
目的
比较第5天囊胚移植与第2天胚胎移植的结局。
方法
将接受卵巢超刺激和卵母细胞采集的不孕女性进行分组：第1组为第2天胚胎移植，第2组在无血清序贯培养基中培养至第5天进行移植。检测早期胚胎质量和生长情况、囊胚形成及质量、着床率（IR）和妊娠率（PR）。
结果
囊胚总形成率为49.4%。早期胚胎质量更好（第2、3天）且第3天的卵裂球数量更多，囊胚形成率更高。第2天和第5天胚胎的着床率分别为10.8%和22.2%。两组的妊娠率相当（37.3%对41.8%）。
结论
囊胚移植的着床率高于第2天胚胎移植，妊娠率相当。早期胚胎质量和第3天的卵裂球数量有助于预测最终的囊胚形成。囊胚形成率与产妇年龄、不孕原因、授精方式及早期胚胎数量无关。
Zotero 插件