Center for Environment and Water Resources, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
Center for Environment and Water Resources, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
Food Chem. 2018 Sep 15;260:174-182. doi: 10.1016/j.foodchem.2018.04.007. Epub 2018 Apr 5.
A new, sensitive, and rapid method based on the Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) approach and air-assisted liquid-liquid microextraction (AALLME) technology was developed for the determination of 20 endocrine-disrupting compounds (EDCs) in fish by high-performance liquid chromatography-tandem mass spectrometry. The method first integrates AALLME into QuEChERS to achieve clean-up and enrichment of the EDCs in one step. A self-made glass tube was enfolded with plasticine to withstand the high centrifugal force. The established method was developed by optimization of the parameters. High linearities (R > 0.9924) and recoveries (78.2-118.6%) at three spiked levels (5, 10, and 20 ng g), and low relative standard deviation values (1.1-14.5%) and limits of detection (0.03-0.80 ng g) were obtained. The method comparison shows that the proposed method is superior as it involves less organic solvent usage, simple operation and high efficiency. This method was successfully applied to different fishes for analyzing EDCs.
基于快速、简便、价廉、有效、稳定和安全（QuEChERS）方法和空气辅助液-液微萃取（AALLME）技术，开发了一种新的、灵敏的、快速的方法，用于通过高效液相色谱-串联质谱法测定鱼类中的 20 种内分泌干扰化合物（EDCs）。该方法首先将 AALLME 集成到 QuEChERS 中，实现了 EDCs 的一步净化和富集。自制的玻璃管用橡皮泥包裹，以承受高离心力。通过优化参数建立了该方法。在三个加标水平（5、10 和 20ng/g）下，获得了高线性（R>0.9924）和回收率（78.2-118.6%），以及低相对标准偏差值（1.1-14.5%）和检测限（0.03-0.80ng/g）。方法比较表明，该方法具有有机溶剂用量少、操作简单、效率高等优点。该方法成功应用于不同鱼类中分析 EDCs。
