Dolling Sophie, Reis-Santos Patrick, Williams Mike, Gillanders Bronwyn M
Southern Seas Ecology Laboratory, School of Biological Sciences and Environment Institute, University of Adelaide, Adelaide, South Australia, Australia.
Environment, CSIRO Environment, Waite Campus, Adelaide, South Australia, Australia.
J Sep Sci. 2025 Feb;48(2):e70084. doi: 10.1002/jssc.70084.
The rise of plastic pollution in marine environments has been heavily documented, with particular focus on the physical impacts the plastics can have on biota. But, plastics also sorb a range of hydrophobic chemical pollutants, acting as vectors for the transportation of these compounds throughout marine environments. Therefore, an analytical method that can target both marine biota and plastic matrices will be key to advance our understanding of the link between chemicals in the environment, plastic pollution, and effects on biota. Here, an efficient method for the detection and quantification of a broad suite of compounds in marine samples was developed. Five extraction methods were trialed for the analysis of 21 pesticides, PFAS, and pharmaceuticals in biota and plastics. This included three ultrasonic extraction methods and two QuEChERS methods. Ultrasonic extraction in acetonitrile with a microcentrifuge step then concentration by Bond Elut Carbon SPE resulted in best recovery across most compounds. Of the 21 compounds trialed, 16 were efficiently quantified. Method limits of quantification and detection were between 0.02 and 4.81 ppb (mLODs) and between 0.06 and 14.60 ppb (mLOQs). This method is widely applicable to a range of marine environments and supports routine evaluations of environmental safety and monitoring protocols.
海洋环境中塑料污染的增加已有大量文献记载，尤其关注塑料对生物群可能产生的物理影响。但是，塑料也会吸附一系列疏水性化学污染物，成为这些化合物在整个海洋环境中传输的载体。因此，一种能够同时针对海洋生物群和塑料基质的分析方法对于推进我们对环境中化学物质、塑料污染以及对生物群影响之间联系的理解至关重要。在此，开发了一种用于检测和定量海洋样品中一系列化合物的有效方法。对五种提取方法进行了试验，以分析生物群和塑料中的21种农药、全氟辛烷磺酸及相关物质和药物。这包括三种超声提取方法和两种QuEChERS方法。在乙腈中进行超声提取并经过微量离心步骤，然后通过Bond Elut Carbon固相萃取进行浓缩，在大多数化合物中回收率最佳。在试验的21种化合物中，有16种得到了有效定量。方法的定量限和检测限分别在0.02至4.81 ppb（方法检测限）和0.06至14.60 ppb（方法定量限）之间。该方法广泛适用于一系列海洋环境，并支持对环境安全性和监测方案的常规评估。
