School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, PR China.
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, PR China.
Food Chem. 2018 Sep 15;260:66-72. doi: 10.1016/j.foodchem.2018.03.062. Epub 2018 Mar 15.
An efficient method was developed and validated for simultaneous determination of tebuconazole, thiophanate-methyl and its metabolite carbendazim in grape and soil using QuEChERS procedure and liquid chromatography-tandem mass spectrometry. The average recoveries of the method were 83.2%-105.4%, the limit of detection (LOD) ranged from 2.1 × 10 to 8.7 × 10 mg L, and the limit of quantitation (LOQ) was 0.1 mg kg. The field results showed that tebuconazole and thiophanate-methyl in soil and grape were rapidly dissipated with half-lives less than 24.7 days. The terminal residues of all analytes in grape were lower than the maximum residue limit (MRL) set by China (2 mg kg for tebuconazole; 3 mg kg for both thiophanate-methyl and carbendazim). Risk assessment showed that tebuconazole, thiophanate-methyl and its metabolite would be unlikely to cause health problems. However, carbendazim, the higher active metabolites of thiophanate-methyl, should receive more concerns.
建立并验证了一种采用 QuEChERS 方法和液相色谱-串联质谱法同时测定葡萄和土壤中戊唑醇、噻菌灵及其代谢物多菌灵的分析方法。该方法的平均回收率为 83.2%105.4%，检测限（LOD）范围为 2.1×108.7×10 mg/L，定量限（LOQ）为 0.1 mg/kg。田间试验结果表明，戊唑醇和噻菌灵在土壤和葡萄中的消解较快，半衰期均小于 24.7 天。所有分析物在葡萄中的最终残留量均低于中国设定的最大残留限量（MRL）（戊唑醇 2 mg/kg；噻菌灵和多菌灵均为 3 mg/kg）。风险评估表明，戊唑醇、噻菌灵及其代谢物不太可能对健康造成危害。然而，噻菌灵的高活性代谢物多菌灵应引起更多关注。
