Institut Galien Paris-Sud, CNRS, Université Paris-Sud, Université Paris-Saclay, 5 rue Jean-Baptiste Clément, 92290, Châtenay-Malabry, France.
CNRS UMR 8612, Institut Galien Paris Sud, Université Paris-Sud, Université Paris-Saclay, 5, rue Jean-Baptiste Clément, 92296, Châtenay-Malabry, France.
Pharm Res. 2018 Apr 26;35(7):129. doi: 10.1007/s11095-018-2406-5.
A Surface Plasmon Resonance chip (SPR) was developed to study the activation of complement system triggered by nanomaterials in contact with human serum, which is an important concern today to warrant safety of nanomedicines.
The developed chip was tested for its specificity in complex medium and its longevity of use. It was then employed to assess the release of complement fragments upon incubation of nanoparticles in serum. A comparison was made with other current methods assessing complement activation (μC-IE, ELISA).
The SPR chip was found to give a consistent response for C3a release upon activation by nanoparticles. Results were similar to those obtained by μC-IE. However, ELISA detection of iC3b fragments showed an explained high non-specific background. The impact of sample preparation preceding the analysis was assessed with the newly develop SPR method. The removal of nanoparticles before analysis showed an important modification in the obtained response, possibly leading to false negative results.
The SPR chip developed in this work allows for an automated assessment of complement activation triggered by nanoparticles with possibility of multiplexed analysis. The design of the chip proved to give consistent results of complement activation by nanoparticles.
开发了一种表面等离子体共振芯片（SPR），用于研究纳米材料与人血清接触时引发的补体系统激活，这是当今保证纳米药物安全性的一个重要关注点。
测试了所开发的芯片在复杂介质中的特异性及其使用的耐久性。然后，将其用于评估纳米颗粒在血清中孵育时释放的补体片段。与其他当前评估补体激活的方法（μC-IE、ELISA）进行了比较。
SPR 芯片在被纳米颗粒激活时对 C3a 释放的反应一致。结果与 μC-IE 获得的结果相似。然而，ELISA 检测 iC3b 片段显示出可解释的高非特异性背景。在分析前评估样品制备的影响，用新开发的 SPR 方法进行。在分析前去除纳米颗粒会导致获得的反应发生重要改变，可能导致假阴性结果。
本工作中开发的 SPR 芯片允许自动评估纳米颗粒引发的补体激活，具有多重分析的可能性。芯片的设计被证明可提供一致的纳米颗粒引发补体激活的结果。
