Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 050095 Bucharest, Romania.
Institut de Chimie Moléculaire de Reims, CNRS UMR 7312, Université de Reims Champagne-Ardenne URCA, CEDEX 2, F-51685 Reims, France.
Int J Mol Sci. 2022 Mar 17;23(6):3258. doi: 10.3390/ijms23063258.
The aim of this study was to investigate the biocompatibility of contrast agents, such as gadolinium 1, 4, 7, 10 tetraazacyclo-dodecane tetraacetic acid (GdDOTA) and gadolinium dioctyl terephthalate (GdDOTP), encapsulated in a polymeric matrix containing chitosan and hyaluronic acid using RAW264.7 murine macrophages and human blood samples. The cell viability and cytotoxicity were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays, while cell cycle analysis was determined in RAW264.7 cells using flow cytometry. The mitochondrial membrane potential (MMP), hemolytic index, complement activation, and thrombogenic potential of gadolinium (Gd) containing nanohydrogels were measured by fluorometric and spectrophotometric methods. Taken together, our results demonstrate the good bio- and hemocompatibility of chitosan-based nanohydrogels with the RAW264.7 cell line and human blood cells, suggesting that these could be used as injectable formulations for the magnetic resonance imaging diagnostic of lymph nodes.
本研究旨在探讨造影剂的生物相容性，如包裹在含有壳聚糖和透明质酸的聚合物基质中的钆 1、4、7、10 四氮杂环十二烷四乙酸（GdDOTA）和二辛基邻苯二甲酸钆（GdDOTP），使用 RAW264.7 鼠巨噬细胞和人血样本进行评估。通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）和乳酸脱氢酶（LDH）测定法评估细胞活力和细胞毒性，而通过流式细胞术在 RAW264.7 细胞中测定细胞周期分析。通过荧光和分光光度法测量含钆纳米水凝胶的线粒体膜电位（MMP）、溶血指数、补体激活和血栓形成潜力。综上所述，我们的结果表明，基于壳聚糖的纳米水凝胶具有良好的生物相容性和血液相容性，与 RAW264.7 细胞系和人血细胞一起使用，可作为用于淋巴结磁共振成像诊断的可注射制剂。
