Department of Chemical Engineering, Federal University of Santa Maria, 1000, Roraima Avenue, Santa Maria, RS, 97105-900, Brazil.
Department of Chemical and Food Engineering, Federal University of Santa Catarina, Caixa Postal 476, Florianópolis, SC, 88040-900, Brazil.
Bioprocess Biosyst Eng. 2018 Aug;41(8):1185-1193. doi: 10.1007/s00449-018-1947-7. Epub 2018 Apr 26.
This work investigates the use of blends of edible and nonedible raw materials as an alternative feedstock to fatty acid methyl esters (FAME) production through enzymatic catalysis. As biocatalyst, liquid lipase from Thermomyces lanuginosus (Callera™ Trans L), was used. Under reaction conditions of 35 °C, methanol to feedstock molar ratio of 4.5:1 and 1.45% of catalyst load, the best process performance was reached using 9% of water concentration in the medium-yield of 79.9% after 480 min of reaction. In terms of use of tallow mixed with soybean oil, the best yield was obtained when 100% of tallow was used in the process-84.6% after 480 min of reaction-behavior that was associated with the degree of unsaturation of the feedstock, something by that time, not addressed in papers of the area. The results show that tallow can be used as an alternative to FAME production, catalyzed by soluble lipase.
本工作研究了通过酶催化将可食用和不可食用的原料混合物用作脂肪酸甲酯 (FAME) 生产的替代原料。液体脂肪酶来自Thermomyces lanuginosus（Callera™ Trans L）被用作生物催化剂。在 35°C 的反应条件下，甲醇与原料的摩尔比为 4.5:1，催化剂负载量为 1.45%，在介质中含水量为 9%的最佳工艺条件下，反应 480 分钟后产率最高，为 79.9%。就牛脂与大豆油混合使用而言，当过程中 100%使用牛脂时，获得了最佳的产率-84.6%，反应 480 分钟-这种行为与原料的不饱和程度有关，而这在该领域的论文中尚未涉及。结果表明，脂可以作为 FAME 生产的替代物，由可溶脂肪酶催化。
