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曲霉属（Aspergillus）tubingensis G131 的全基因组测序及次生代谢产物潜能概述。

Whole-genome sequencing of Aspergillus tubingensis G131 and overview of its secondary metabolism potential.

机构信息

Université de Toulouse, Laboratoire de Génie Chimique, UMR 5503 CNRS/INPT/UPS, INP-ENSAT, 1, avenue de l'Agrobiopôle, 31326, Castanet-Tolosan, France.

Present address: Unité de Recherche Biologie des Plantes et Innovation (BIOPI-EA 3900), Université de Picardie Jules Verne, 33 rue Saint Leu, 80039, Amiens Cedex, France.

出版信息

BMC Genomics. 2018 Mar 15;19(1):200. doi: 10.1186/s12864-018-4574-4.

DOI:10.1186/s12864-018-4574-4
PMID:29703136
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6389250/
Abstract

BACKGROUND

Black Aspergilli represent one of the most important fungal resources of primary and secondary metabolites for biotechnological industry. Having several black Aspergilli sequenced genomes should allow targeting the production of certain metabolites with bioactive properties.

RESULTS

In this study, we report the draft genome of a black Aspergilli, A. tubingensis G131, isolated from a French Mediterranean vineyard. This 35 Mb genome includes 10,994 predicted genes. A genomic-based discovery identifies 80 secondary metabolites biosynthetic gene clusters. Genomic sequences of these clusters were blasted on 3 chosen black Aspergilli genomes: A. tubingensis CBS 134.48, A. niger CBS 513.88 and A. kawachii IFO 4308. This comparison highlights different levels of clusters conservation between the four strains. It also allows identifying seven unique clusters in A. tubingensis G131. Moreover, the putative secondary metabolites clusters for asperazine and naphtho-gamma-pyrones production were proposed based on this genomic analysis. Key biosynthetic genes required for the production of 2 mycotoxins, ochratoxin A and fumonisin, are absent from this draft genome. Even if intergenic sequences of these mycotoxins biosynthetic pathways are present, this could not lead to the production of those mycotoxins by A. tubingensis G131.

CONCLUSIONS

Functional and bioinformatics analyses of A. tubingensis G131 genome highlight its potential for metabolites production in particular for TAN-1612, asperazine and naphtho-gamma-pyrones presenting antioxidant, anticancer or antibiotic properties.

摘要

背景

黑曲霉是生物技术产业中最重要的初级和次级代谢产物的真菌资源之一。拥有几个黑曲霉测序基因组应该可以使某些具有生物活性的代谢产物的生产成为可能。

结果

在这项研究中，我们报告了从法国地中海葡萄园分离的黑曲霉 A. tubingensis G131 的草图基因组。这个 35 Mb 的基因组包含 10994 个预测基因。基于基因组的发现鉴定了 80 个次级代谢产物生物合成基因簇。这些簇的基因组序列在 3 个选择的黑曲霉基因组上进行了blast：A. tubingensis CBS 134.48、A. niger CBS 513.88 和 A. kawachii IFO 4308。这种比较突出了这四个菌株之间簇的不同程度的保守性。它还可以鉴定出 A. tubingensis G131 中七个独特的簇。此外，基于这种基因组分析，提出了 Asperazine 和 Naphtho-Gamma-Pyrones 生产的假定次级代谢物簇。该草案基因组中不存在生产两种真菌毒素，即赭曲霉毒素 A 和伏马菌素所需的关键生物合成基因。即使这些真菌毒素生物合成途径的基因间序列存在，也不能导致 A. tubingensis G131 产生这些真菌毒素。

结论

A. tubingensis G131 基因组的功能和生物信息学分析突出了其在代谢产物生产方面的潜力，特别是 TAN-1612、Asperazine 和 Naphtho-Gamma-Pyrones，它们具有抗氧化、抗癌或抗生素特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0ef/6389250/707f62a7da9a/12864_2018_4574_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0ef/6389250/1f01456f5f55/12864_2018_4574_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0ef/6389250/09765e4646d4/12864_2018_4574_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0ef/6389250/d4b3a634f64d/12864_2018_4574_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0ef/6389250/2b15b2944c85/12864_2018_4574_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0ef/6389250/707f62a7da9a/12864_2018_4574_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0ef/6389250/1f01456f5f55/12864_2018_4574_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0ef/6389250/09765e4646d4/12864_2018_4574_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0ef/6389250/d4b3a634f64d/12864_2018_4574_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0ef/6389250/2b15b2944c85/12864_2018_4574_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0ef/6389250/707f62a7da9a/12864_2018_4574_Fig5_HTML.jpg

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