Reimer L, Reller B, Wang W L, Mirrett S
Department of Pathology, Veterans Administration Medical Center, Salt Lake City, Utah 84148.
Eur J Clin Microbiol Infect Dis. 1988 Jun;7(3):384-7. doi: 10.1007/BF01962342.
In vitro, 1.2% gelatin counteracts the inhibition of growth of bacterial species by sodium polyanetholsulfonate in blood culture media. Additionally, 1% yeast extract has been used to promote bacterial growth. We compared the performance of supplemented peptone broth and Trypticase soy broth, both of which contained sodium polyanetholsulfonate, gelatin and yeast extract. Trypticase soy broth with gelatin and yeast extract inhibited (p less than 0.001) and delayed growth, especially of gram-positive (p less than 0.01) and gram-negative (p less than 0.005) anaerobic bacteria. Although the recovery of organisms usually inhibited by sodium polyanetholsulfonate was similar in supplemented peptone and Trypticase soy broths, supplemented peptone broth clearly was superior in the recovery of other organisms commonly found in blood cultures.
在体外，1.2%的明胶可抵消血液培养基中聚茴香脑磺酸钠对细菌生长的抑制作用。此外，1%的酵母提取物已被用于促进细菌生长。我们比较了添加蛋白胨肉汤和胰蛋白酶大豆肉汤的性能，这两种肉汤均含有聚茴香脑磺酸钠、明胶和酵母提取物。含有明胶和酵母提取物的胰蛋白酶大豆肉汤会抑制（p<0.001）并延迟生长，尤其是革兰氏阳性（p<0.01）和革兰氏阴性（p<0.005）厌氧菌。尽管在添加蛋白胨肉汤和胰蛋白酶大豆肉汤中，通常受聚茴香脑磺酸钠抑制的微生物的回收率相似，但添加蛋白胨肉汤在血液培养中常见的其他微生物的回收率方面明显更具优势。
