Tenney J H, Reller L B, Mirrett S, Wang W L, Weinstein M P
J Clin Microbiol. 1982 Apr;15(4):558-61. doi: 10.1128/jcm.15.4.558-561.1982.
To evaluate the role of the volume of blood cultured in the detection of clinically important bacteremia and fungemia in adults, we evaluated the yield and speed of detection of microorganisms from 5,317 paired 2- and 5-ml samples of blood. The same kind of medium (supplemented peptone broth with 0.03% sodium polyanetholsulfonate) and atmosphere of incubation (open venting units) were used for all blood cultures. Only adequately filled (less than or equal to 80% of stated volume) sets (20-ml tube and 50-ml bottle) were compared statistically. Significantly more bacteria (p less than 0.01), Pseudomonas spp. In particular (P less than 0.05), were isolated from the 5-ml samples of blood. We conclude that the volume of blood cultured is a critical factor in the detection of septicemia. Consequently, valid evaluation of other factors influencing the detection of septicemia must be based on comparisons in which equal volumes of blood are cultured.
为评估成人血培养体积在检测临床重要菌血症和真菌血症中的作用，我们评估了从5317对2毫升和5毫升血液样本中检测微生物的产量和速度。所有血培养均使用同一种培养基（添加0.03%聚茴香脑磺酸钠的蛋白胨肉汤）和孵育环境（开放式通风装置）。仅对充分填充（小于或等于规定体积的80%）的培养瓶（20毫升试管和50毫升瓶子）进行统计学比较。从5毫升血液样本中分离出的细菌显著更多（p<0.01），尤其是假单胞菌属（P<0.05）。我们得出结论，血培养体积是检测败血症的关键因素。因此，对影响败血症检测的其他因素进行有效评估必须基于培养相同体积血液的比较。
